NDLI: Electrochemical detection of in situ DNA damage induced by enzyme-catalyzed Fenton reaction. Part II in hydrophobic room temperature ionic liquid

Content Provider	Springer Nature Link
Author	Chen, Yang Xiong, Huayu Zhang, Xiuhua Wang, Shengfu
Copyright Year	2012
Abstract	A hydrophobic room temperature ionic liquid, 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM][PF6]), was applied as nonaqueous solvent for the generation of hydroxy radical (•OH) through glucose oxidase-catalyzed Fenton reaction. The enzyme catalyzes the oxidation of glucose, and the produced H2O2 further reacts with transition metal ions, generating hydroxyl radicals. They attacked DNA and led its damage. This was detected by square wave voltammetry (SWV) of the electroactive indicator Co(bpy) 3 3+ . It bound more strongly to intact DNA, and the SWV peak currents decreased at the potential of 0.064 V when DNA was damaged. The experimental results testified that the antioxidants, ascorbic acid, aloe-emodin and rutin, inhibited oxidative DNA damage by hydroxyl radicals. The method is promising for rapid, sensitive, and inexpensive detection of DNA damage. Figure Schematic diagram for working principle of SWV detection of in situ DNA damage for DNA-GOx film.
Starting Page	45
Ending Page	51
Page Count	7
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	178
Issue Number	1-2
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2012-04-05
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	DNA damage Glucose oxidase Room temperature ionic liquid Co(bpy) 3 3+ Hydroxyl radical Analytical Chemistry Nanochemistry Characterization and Evaluation of Materials Microengineering Nanotechnology
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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