NDLI: Mediatorless amperometric bienzyme glucose biosensor based on horseradish peroxidase and glucose oxidase cross-linked to multiwall carbon nanotubes

Content Provider	Springer Nature Link
Author	Xu, Shuxia Qi, Honglan Zhou, Shiyi Zhang, Xinfeng Zhang, Chengxiao
Copyright Year	2014
Abstract	We report on a bienzyme-channeling sensor for sensing glucose without the aid of mediator. It was fabricated by cross-linking horseradish peroxidase (HRP) and glucose oxidase (GOx) on a glassy carbon electrode modified with multiwalled carbon nanotubes (MWNTs). The bienzyme was cross-linked with the MWNTs by glutaraldehyde and bovine serum albumin. The MWNTs were employed to accelerate the electron transfer between immobilized HRP and electrode. Glucose was sensed by amperometric reduction of enzymatically generated H2O2 at an applied voltage of −50 mV (vs. Ag/AgCl). Factors influencing the preparation and performance of the bienzyme electrode were investigated in detail. The biosensor exhibited a fast and linear response to glucose in the concentration range from 0.4 to 15 mM, with a detection limit of 0.4 mM. The sensor exhibited good selectivity and durability, with a long-term relative standard deviation of <5 %. Analysis of glucose-spiked human serum samples yielded recoveries between 96 and 101 %. Figure A novel bienzyme-channeling sensor for glucose sensing has been constructed without the aid of mediator. This biosensor was fabricated by cross-linking horseradish peroxidase (HRP) and glucose oxidase (GOD) onto glass carbon electrode (GCE) modified with multiwall carbon nanotubes (MWNTs) which accelerated the electron transfer between the HRP and electrode.
Starting Page	535
Ending Page	541
Page Count	7
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	181
Issue Number	5-6
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2014-01-30
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Amperometric bienzyme biosensor Multiwall carbon nanotubes Cross-linking Mediatorless Glucose Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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