NDLI: ‘Green’ immunochromatographic electrochemical biosensor for mercury(II)

Content Provider	Springer Nature Link
Author	Wang, Yulong Wang, Limin Wang, Suyan Yang, Mingming Cai, Jia Liu, Fengquan
Copyright Year	2016
Abstract	We describe an immunochromatographic electrochemical biosensor (IEB) for highly specific and sensitive determination of Hg(II) ions. The IEB is based on the use of a new monoclonal antibody (McAb) against Hg(II) ions that affects the recognition of an antigen. The McAb is placed on the surface of gold nanoparticles (AuNPs) and can recognize the antigen only in the absence of Hg(II) ions. This detection scheme was used to design an immunochromatographic test strip using dually labeled AuNPs along with electrochemical detection. Signal amplification was accomplished by a competitive reaction and the use of horseradish peroxidase. Following immunochromatography, the test zone was cut out and transferred into a reaction cell loaded with a substrate solution containing ortho-phenylenediamine and H2O2. After 10-min incubation with horseradish peroxidase, square wave voltammetry was performed with a screen-printed electrode. Under optimal conditions and a working voltage of −0.57 V, the IEB displays a linear response in the 0.1 to 200 ng.mL−1 Hg(II) concentration range and a 30 pg.mL−1 limit of detection. It was applied to the determination of Hg(II) in (spiked) waters and milk where its sensitivity by far surpassed the maximum allowed contamination levels. This sensitive IEB therefore possesses substantial advantages over other assays. In addition, the detection scheme may be extended to other metal ions for which appropriate antibodies are available. Graphical abstract We developed an immunochromatographic electrochemical biosensor (IEB) for highly specific and sensitive determination of Hg(II) ions in water and milk by using a new anti-Hg2+ monoclonal antibody (McAb). The linear range and limit of detection is 0.1–200 ng·mL−1 and 30 pg.mL−1, respectively.
Starting Page	2509
Ending Page	2516
Page Count	8
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	183
Issue Number	9
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2016-05-16
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Test stripe Immunochromatography Lateral flow assay Heavy metal Stripping wave voltammetry Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Simultaneous determination of cadmium(II), lead(II) and copper(II) by using a screen-printed electrode modified with mercury nano-droplets

Centri-voltammetry and biocentri-voltammetry: a review

Square wave anodic stripping voltammetry of copper(II) at a MWCNT paste electrode modified with a tetracarbonylmolybdenum(0) nanocomposite

Electrochemical simultaneous assay of chloramphenicol and PCB72 using magnetic and aptamer-modified quantum dot-encoded dendritic nanotracers for signal amplification

Stripping voltammetric determination of mercury(II) and lead(II) using screen-printed electrodes modified with gold films, and metal ion preconcentration with thiol-modified magnetic particles

Highly sensitive uric acid biosensor based on individual zinc oxide micro/nanowires

Screen-printed electrodes made of a bismuth nanoparticle porous carbon nanocomposite applied to the determination of heavy metal ions

Enzyme-free electrochemical detection of microRNA-21 using immobilized hairpin probes and a target-triggered hybridization chain reaction amplification strategy

A glassy carbon electrode modified with antimony and poly(p-aminobenzene sulfonic acid) for sensing lead(II) by square wave anodic stripping voltammetry

‘Green’ immunochromatographic electrochemical biosensor for mercury(II)

Similar Documents

Simultaneous determination of cadmium(II), lead(II) and copper(II) by using a screen-printed electrode modified with mercury nano-droplets

Centri-voltammetry and biocentri-voltammetry: a review

Square wave anodic stripping voltammetry of copper(II) at a MWCNT paste electrode modified with a tetracarbonylmolybdenum(0) nanocomposite

Electrochemical simultaneous assay of chloramphenicol and PCB72 using magnetic and aptamer-modified quantum dot-encoded dendritic nanotracers for signal amplification

Stripping voltammetric determination of mercury(II) and lead(II) using screen-printed electrodes modified with gold films, and metal ion preconcentration with thiol-modified magnetic particles

Highly sensitive uric acid biosensor based on individual zinc oxide micro/nanowires

Screen-printed electrodes made of a bismuth nanoparticle porous carbon nanocomposite applied to the determination of heavy metal ions

Enzyme-free electrochemical detection of microRNA-21 using immobilized hairpin probes and a target-triggered hybridization chain reaction amplification strategy

A glassy carbon electrode modified with antimony and poly(p-aminobenzene sulfonic acid) for sensing lead(II) by square wave anodic stripping voltammetry

‘Green’ immunochromatographic electrochemical biosensor for mercury(II)