NDLI: A magnetic relaxation switch aptasensor for the rapid detection of Pseudomonas aeruginosa using superparamagnetic nanoparticles

Content Provider	Springer Nature Link
Author	Jia, Fei Xu, Lei Yan, Wenjie Wu, Wei Yu, Qianqian Tian, Xiaojing Dai, Ruitong Li, Xingmin
Copyright Year	2017
Abstract	The authors describe a rapid and sensitive magnetic relaxation switch (MRSw) aptasensor for the determination of the food-borne pathogen Pseudomonas aeruginosa. An aptamer against P. aeruginosa is covalently bound to superparamagnetic iron oxide nanoparticles. On incubation with P. aeruginosa bacteria, they will be captured by the aptamer, and this affects the formation of SPIO aggregates. The resulting strong increase in the spin-spin relaxation time (T2) is utilized as the analytical information to quantify the bacteria. Under optimized conditions, the assay has a linear range that extends from 100 cfu⋅mL−1 to 106 cfu⋅mL−1, and a detection limit of 50 cfu⋅mL−1 (at an S/N ratio of 3). The method was applied to the detection of P. aeruginosa in (spiked) real food and drinking water samples and was validated by the established plate counting method. This aptasensor is considered to represent a promising platform for the determination of P. aeruginosa. Conceivably, the method may be extended to other food-borne bacteria or biomolecules for which respective aptamers are available. Graphical abstract Schematic of the magnetic relaxation switch aptasensor for Pseudomonas aeruginosa detection. The superparamagnetic nanoparticles act as a “switch” to detect aggregated and dispersed states with and without target, which leads to substantial changes in the T2 relaxation time.
Starting Page	1539
Ending Page	1545
Page Count	7
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	184
Issue Number	5
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2017-03-10
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Transverse relaxation time Carr-Purcell-Meiboom-Gill pulse sequence Foodborne pathogen Particle aggregation Low-field NMR Meat spoilage Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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