NDLI: Electrochemiluminescent detection of Hg(II) by exploiting the differences in the adsorption of free T-rich oligomers and Hg(II) loaded T-rich oligomers on silica nanoparticles doped with Ru(bpy)3 2+

Content Provider	Springer Nature Link
Author	Cai, Linfang Guo, Zhihui Zheng, Xingwang
Copyright Year	2016
Abstract	The detection scheme for Hg(II) ions described here is based on the finding that nanoparticles (NPs) prepared from silica and chitosan, and doped with the ruthenium(II) bipyridyl complex [Ru(bpy)3 2+] display large differences in their affinity for T-rich oligomers and Hg(II)-loaded T-rich oligomers. The effect was exploited to design a sensitive electrochemiluminescence (ECL) based assay for Hg(II). In the absence of Hg(II), the oligomers (with 40 bases) strongly bind to the surface of the NPs. Such oligomer-loaded NPs do not bind to a glass carbon electrode (GCE) modified with carbon nanotubes (CNT) in a nafion host matrix. If, however, Hg(II) is present, it will cause the formation of Hg(II)-loaded double-stranded oligomers via T-Hg-T duplexes which are not easily adsorbed on the doped silica NPs. Such NPs are readily assembled on the GCE and this will result in strong ECL. The increase in ECL intensity is a viable parameter for detection of Hg(II) with a detection limit as low as 0.3 pM. Such as sensitivity has not been accomplished in previously reported ECL based methods for detecting Hg(II). In our perception, the method based on differential adsorption presented here is not limited to the detection of Hg(II) but represents a method of wider scope in that other analytes may be detected by this method if respective oligomer probes are available. Graphical abstract An electrochemiluminescence (ECL) based method is described for determination of Hg(II). It is based on the differential adsorption effect of ECL-active carbon nanotube (CNT) nanoparticles towards a DNA oligonucleotide probe and its complex with Hg(II).
Starting Page	2345
Ending Page	2351
Page Count	7
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	183
Issue Number	7
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2016-05-26
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Hg(II) binding DNA probe Nanomaterials Chitosan Carbon nanotubes Microemulsion Tetraethylorthosilicate Transmission electron microscopy Polyacrylamide gel electrophoresis Circular dichroism T-Hg-T duplex Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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