NDLI: Immunoassay for serum amyloid A using a glassy carbon electrode modified with carboxy-polypyrrole, multiwalled carbon nanotubes, ionic liquid and chitosan

Content Provider	Springer Nature Link
Author	Xia, Chunyong Li, Yuan Yuan, Guolin Guo, Yanlei Yu, Chao
Copyright Year	2015
Abstract	We report on a highly sensitive electrochemical immunoassay for the serum inflammation marker amyloid A (SAA). It is making use of a glassy carbon electrode that was modified with carboxy-endcapped polypyrrole (PPy-α-COOH), multiwalled carbon nanotubes (MWCNTs), ionic liquid and chitosan acting as the support platform. The nanocomposite increases the sensitivity and stability of the assay. Antibody against SAA was immobilized on a monolayer surface consisting of PPy-α-COOH. The electrode material was characterized by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectra, cyclic voltammetry, electrochemical impedance spectroscopy and differential pulse voltammetry. The calibration plot for this assay, when operated at 0.16 V (vs. SCE) and applied to spiked serum samples, is linear in the 0.001 to 900 ng mL−1 SAA concentration range, and the detection limit is as low as 0.3 pg mL−1 (at an S/N ratio of 3). The electrode is stable and highly sensitive. The detection scheme is likely to be applicable to numerous other kinds of immunoassays. Graphical Abstract We describe a highly sensitive serum amyloid A (SAA) electrochemical immunosensor based on in situ polymerization of carboxy-endcapped polypyrrole (PPy-α-COOH) on multiwalled carbon nanotubes (MWCNTs), ionic liquid (IL), and chitosan (Chit) nanocomposite. Excellent performance is achieved because PPy-α-COOH offers anchor sites for covalent immobilization of anti-SAA, while the MWCNTS/IL/Chit nanocomposite warrants good electrical conductivity and high stability.
Starting Page	1395
Ending Page	1402
Page Count	8
File Format	PDF
ISSN	00263672
Journal	Microchimica Acta
Volume Number	182
Issue Number	7-8
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2015-03-05
Publisher Place	Vienna
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Carboxy-endcapped polypyrrole Ionic liquid Multiwalled carbon nanotubes Chitosan Serum amyloid A Nanochemistry Nanotechnology Characterization and Evaluation of Materials Analytical Chemistry Microengineering
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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