NDLI: Comparison of morphological and molecular genetic quantification of relative abundance of arbuscular mycorrhizal fungi within roots

Content Provider	Springer Nature Link
Author	Shi, P. Abbott, L. K. Banning, N. C. Zhao, B.
Copyright Year	2012
Abstract	Nested PCR amplicons of ribosomal RNA genes have been used to identify individuals within assemblages of arbuscular mycorrhizal (AM) fungi in roots and to estimate their relative abundance. Microscopy has also been used to identify their relative abundance in roots, but only at low resolution, usually the genus level. We evaluated the robustness of using nested PCR amplicons of ribosomal RNA genes to estimate the relative abundance of undefined AM fungi in uniformly aged roots in comparison to visual estimates. The relative abundance of AM fungi was assessed as per cent root length colonised by morphotypes and relative sequence type abundance in clone libraries. Plants were grown in coastal soil to obtain assemblages of unknown AM fungi at two times (spring and autumn). Relative abundance of dominant genera of AM fungi in roots (Archaeospora and Glomus) based on an analysis of ribosomal RNA genes did not consistently correspond with relative abundance of morphotypes. This microscopic vs. molecular genetic comparison supports previous conclusions that there can be limitations in using nested PCR amplicons for quantifying the relative abundance of AM fungi in roots, with a sampling bias likely to be of significance. Both molecular genetic and morphological methods are used to estimate relative abundance of AM fungi as a precursor to understanding mycorrhizal function in field soils, but they are rarely verified using alternative approaches although this may be necessary.
Starting Page	501
Ending Page	513
Page Count	13
File Format	PDF
ISSN	09406360
Journal	Mycorrhiza
Volume Number	22
Issue Number	7
e-ISSN	14321890
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-01-10
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Glomeromycota Clone library Microscopy Acacia cochlearis Olearia axillaris Agriculture Forestry Plant Sciences Microbiology Ecology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Medicine Molecular Biology Ecology, Evolution, Behavior and Systematics

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