NDLI: A qPCR assay that specifically quantifies Tricholoma matsutake biomass in natural soil

Content Provider	Springer Nature Link
Author	Yamaguchi, Muneyoshi Narimatsu, Maki Fujita, Toru Kawai, Masataka Kobayashi, Hisayasu Ohta, Akira Yamada, Akiyoshi Matsushita, rihisa Neda, Hitoshi Shimokawa, Tomoko Murata, Hitoshi
Copyright Year	2016
Abstract	Tricholoma matsutake is an ectomycorrhizal basidiomycete that produces prized, yet uncultivable, “matsutake” mushrooms along densely developed mycelia, called “shiro,” in the rhizosphere of coniferous forests. Pinus densiflora is a major host of this fungus in Japan. Measuring T. matsutake biomass in soil allows us to determine the kinetics of fungal growth before and after fruiting, which is useful for analyzing the conditions of the shiro and its surrounding mycorrhizosphere, predicting fruiting timing, and managing forests to obtain better crop yields. Here, we document a novel method to quantify T. matsutake mycelia in soil by quantifying a single-copy DNA element that is uniquely conserved within T. matsutake but is absent from other fungal species, including close relatives and a wide range of ectomycorrhizal associates of P. densiflora. The targeted DNA region was amplified quantitatively in cultured mycelia that were mixed with other fungal species and soil, as well as in an in vitro co-culture system with P. densiflora seedlings. Using this method, we quantified T. matsutake mycelia not only from shiro in natural environments but also from the surrounding soil in which T. matsutake mycelia could not be observed by visual examination or distinguished by other means. It was demonstrated that the core of the shiro and its underlying area in the B horizon are predominantly composed of fungal mycelia. The fungal mass in the A or A0 horizon was much lower, although many white mycelia were observed at the A horizon. Additionally, the rhizospheric fungal biomass peaked during the fruiting season.
Starting Page	847
Ending Page	861
Page Count	15
File Format	PDF
ISSN	09406360
Journal	Mycorrhiza
Volume Number	26
Issue Number	8
e-ISSN	14321890
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-07-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Edible ectomycorrhizal mushrooms Retrotransposon-associated genomic region Soil fungal biomass Species-specific quantitative PCR Microbiology Plant Sciences Ecology Agriculture Forestry
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Medicine Molecular Biology Ecology, Evolution, Behavior and Systematics

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