NDLI: Microfabricated fluorescence-activated cell sorter through hydrodynamic flow manipulation

Content Provider	Springer Nature Link
Author	Bang, Hyunwoo Chung, Chanil Kim, Jung Kyung Kim, Seong Hwan Chung, Seok Park, Junha Lee, Won Gu Yun, Hoyoung Lee, Joonmo Cho, Keunchang Han, Dong Chul Chang, Jun Keun
Copyright Year	2006
Abstract	In this paper, we present a novel flow manipulation and signal detection scheme for a microfabricated fluorescence-activated cell sorter (μFACS). Optics setup was built on an inverted fluorescence microscope (IX71, Olympus, USA) and microchips were made of PDMS using soft lithography. Perfectly aligned axial illumination setup of the commercial microscope ensures effective signal detection. Hydrodynamic flow manipulation scheme is adopted for both flow focusing and cell sorting. Fast and robust sorting function is utilized by a nozzle flow from wide actuation channel. The sorting function is verified through real-time high-speed imaging. The captured images are used for adjusting delays between signal detection and sorting activation. The system automatically adjusts itself to any flow conditions, and so no adjustment is made to the flow parameters between runs. We believe that the proposed techniques will help breaking through the obstacles of μFACS system development.
Starting Page	746
Ending Page	753
Page Count	8
File Format	PDF
ISSN	09467076
Journal	Microsystem Technologies
Volume Number	12
Issue Number	8
e-ISSN	14321858
Language	English
Publisher	Springer-Verlag
Publisher Date	2006-01-10
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Condensed Matter Physics Electronic, Optical and Magnetic Materials Electrical and Electronic Engineering Hardware and Architecture

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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