NDLI: Coriolis force for facilitating DNA molecular migration and hybridization in compact disk microfluidic platforms

Content Provider	Springer Nature Link
Author	Kassegne, Sam Khosla, Ajit Patel, Dhruv Paramesh, Nithesh Harwood, Nitin Arya, Bhuvnesh
Copyright Year	2014
Abstract	This study investigates the influence of Coriolis force on transport and hybridization of ssDNA molecules in compact disk (CD) microfluidic platform where centrifugal force is used as the driving force. While the effect of Coriolis force on fluid flow in CD microfluidic channels has been studied experimentally and numerically only recently, its influence on ssDNA molecule migration and hybridization has not been investigated so far. This study addresses this phenomenon through numerical simulation and demonstrates that for most practical geometrical configurations and angular velocity ranges reported in the literature, the Coriolis force introduces significant qualitative and quantitative spatial variations in the hybridization of ssDNA molecules, particularly at locations near the periphery. In a particular example investigated here, hybridization was observed to reach steady-state at some locations in about half the time required in the absence of Coriolis force. However, our results further indicate that the time frame for hybridization is so fast (<1 s) that the transient effect due to Coriolis force on the location of hybridization is eventually compensated by the kinetics of hybridization and diffusion-limited reactions that tend to allow a uniform diffusion of target ssDNA over the surface at steady-state. To validate the numerical approach developed here, we carry out several experiments on microfluidic channels in a CD platform. Both numerically and experimentally, Coriolis effect is observed to be significantly influenced by channel width and angular rotations. Our results indicate that for low viscosity fluids, angular velocities as low as 25 rad/s could introduce Coriolis force that is as high as at least 25 % of the main driving centrifugal force.
Starting Page	719
Ending Page	732
Page Count	14
File Format	PDF
ISSN	09467076
Journal	Microsystem Technologies
Volume Number	21
Issue Number	4
e-ISSN	14321858
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-02-11
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Electronics and Microelectronics, Instrumentation Nanotechnology Mechanical Engineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Condensed Matter Physics Electronic, Optical and Magnetic Materials Electrical and Electronic Engineering Hardware and Architecture

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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