NDLI: Generation and analysis of expressed sequence tags from almond (Prunus dulcis Mill.) pistils

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Hormonal status of the pollen-pistil system at the progamic phase of fertilization after compatible and incompatible pollination in Petunia hybrida L.

Generation and analysis of expressed sequence tags from almond (Prunus dulcis Mill.) pistils

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Generation and analysis of expressed sequence tags from almond (Prunus dulcis Mill.) pistils

Content Provider	Springer Nature Link
Author	Jiang, Yuan Qing Ma, Rong Cai
Copyright Year	2003
Abstract	Large-scale single-pass sequencing of expressed sequence tags (ESTs) can give a global picture of the genes involved in the development and function of organs and tissues. Almond (Prunus dulcis Mill. syn. Amygdalus communis L. var. dulcis) is a self-incompatible fruit tree of the family Rosaceae. As an attempt to examine the transcripts expressed during pistil development, we randomly selected and partially sequenced over 1,000 clones from a cDNA library of almond pistils. Analysis of the ESTs using the NCBI non-redundant protein database revealed that 456 (~45.4%) have significant similarity to protein coding sequences in the database. Among the 549 cDNAs with no similarity to known protein sequences, 56 clones have significant similarity to nucleotide sequences registered in the databases. Sequences were assembled using StackPACK and the estimated number of genes identified was 716: 121 contigs and 595 singletons. Only eight of the unique cDNA clones correspond to previously isolated gene sequences of P. dulcis. After functional classification of almond ESTs, "stress resistance and defense" and "protein synthesis and processing" ESTs were found to be the most numerous. Our EST analysis provides a first picture of the numerous almond genes potentially involved in pistil development. It also contributes to our knowledge about gene expression patterns in pistils while providing an extensive reservoir for gene cloning and genetic mapping in almond and other related fruit trees.
Starting Page	197
Ending Page	207
Page Count	11
File Format	PDF
ISSN	09340882
Journal	Plant Reproduction
Volume Number	16
Issue Number	4
e-ISSN	14322145
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-09-23
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Almond Expressed sequence tags Pistil Prunus dulcis Mill.
Content Type	Text
Resource Type	Article
Subject	Cell Biology Plant Science

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Generation and analysis of expressed sequence tags from almond (Prunus dulcis Mill.) pistils

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