NDLI: Enhancement of somatic embryogenesis in camphor tree (Cinnamomum camphora L.): osmotic stress and other factors affecting somatic embryo formation on hormone-free medium

Please wait, while we are loading the content...

Comparative hydraulic and anatomic properties in palm trees (Washingtonia robusta) of varying heights: implications for hydraulic limitation to increased height growth

Short shoots of Betula pendula Roth. as bioindicators of urban environmental pollution in Wrocław (Poland)

In vitro rooting of genetically related Eucalyptus urophylla × Eucalyptus grandis clones in relation to the time spent in culture

No evidence of spatial root system segregation and elevated fine root biomass in multi-species temperate broad-leaved forests

Changes in needle nitrogen partitioning and photosynthesis during 80 years of tree ontogeny in Picea abies

Leaf gas exchange and water potential responses to drought in nine poplar (Populus spp.) clones with contrasting drought tolerance

Warm temperature accelerates short photoperiod-induced growth cessation and dormancy induction in hybrid poplar (Populus × spp.)

Seasonal dimorphism in wood anatomy of the Mediterranean Cistus incanus L. subsp. incanus

Root suckering patterns in Populus euphratica (Euphrates poplar, Salicaceae)

Evidence for the signaling role of methyl jasmonate, methyl salicylate and benzothiazole between poplar (Populus simonii × P. pyramidalis ‘Opera 8277’) cuttings

Developmental and structural features of secretory canals in root and shoot wood of Copaifera langsdorffii Desf. (Leguminosae–Caesalpinioideae)

Large-scale field acclimatization of Olea maderensis micropropagated plants: morphological and physiological survey

Enhancement of somatic embryogenesis in camphor tree (Cinnamomum camphora L.): osmotic stress and other factors affecting somatic embryo formation on hormone-free medium

Laurina mutation affected Coffea arabica tree size and shape mainly through internode dwarfism

Within-branch heterogeneity of the light environment and leaf temperature in a Fagus crenata crown and its significance for photosynthesis calculations

Size mediated climate–growth relationships in Pinus halepensis and Pinus pinea

Genetic diversity and relationships among germplasm of Jatropha curcas L. revealed by RAPDs

Photosynthesis studies on European evergreen and deciduous oaks grown under Central European climate conditions. I: a case study of leaf development and seasonal variation of photosynthetic capacity in Quercus robur (L.), Q. ilex (L.) and their semideciduous hybrid, Q. × turneri (Willd.)

Photosynthesis studies on European evergreen and deciduous oaks grown under Central European climate conditions: II. Photoinhibitory and light-independent violaxanthin deepoxidation and downregulation of photosystem II in evergreen, winter-acclimated European Quercus taxa

Estimation of canopy gap size and gap shape using a hemispherical photograph

Neutron imaging versus standard X-ray densitometry as method to measure tree-ring wood density

Trees : Volume 23, Issue 4, August 2009

Trees : Volume 23, Issue 3, June 2009

Trees : Volume 23, Issue 2, April 2009

Trees : Volume 23, Issue 1, February 2009

Enhancement of somatic embryogenesis in camphor tree (Cinnamomum camphora L.): osmotic stress and other factors affecting somatic embryo formation on hormone-free medium

Content Provider	Springer Nature Link
Author	Shi, Xueping Dai, Xigang Liu, Guofeng Bao, Manzhu
Copyright Year	2009
Abstract	The aim of this study was to improve the direct somatic embryogenesis and initiate embryogenic callus formation in camphor tree (Cinnamomum camphora L.) on hormone-free medium. The influence of osmotic stress pretreatment of immature zygotic embryos (0.5 and 1.0 M solution of sucrose for 12, 24, 48, 72, 96, 120, and 144 h at 4 or 25°C) before cultured on hormone-free medium, on embryogenesis efficiency was assessed. The embryogenesis frequency was improved from 16.29 to 93.27%, while the average number of somatic embryos per explant increased from 3 to 12.57. Activated charcoal (AC), medium renewal, basal medium, light conditions and sucrose concentration in culture medium were also evaluated for their effect on somatic embryogenesis. AC addition and 10-day medium renewal did not increase embryogenesis efficiency significantly, and Murashige and Skoog (MS) medium proved to be more beneficial for somatic embryo formation than others. No differences were found between embryogenesis frequencies when cultured in darkness or under light, but culturing under light yielded more embryos. After the sucrose solution pretreatment, high level concentration of sucrose in induction medium was not needed for somatic embryogenesis, for it had a negative effect on somatic embryo formation when the concentration of sucrose was higher than 50 g l−1. The derived embryogenic lines were maintained via repetitive embryogenesis on hormone-free medium. Low ratio formation of embryogenic callus was observed on the surface of somatic embryos both on induction and proliferation medium. Plantlets derived from somatic embryos grew vigorously with normal appearance similar to germinated zygotic embryos.
Starting Page	1033
Ending Page	1042
Page Count	10
File Format	PDF
ISSN	09311890
Journal	Trees
Volume Number	23
Issue Number	5
e-ISSN	14322285
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-05-22
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cinnamomum camphora L. Hormone-free Osmotic stress Repetitive Somatic embryogenesis Plant Physiology Plant Pathology Plant Anatomy/Development Agriculture Plant Sciences Forestry
Content Type	Text
Resource Type	Article
Subject	Ecology Physiology Plant Science Forestry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Cyclic secondary somatic embryogenesis and efficient plant regeneration in camphor tree (Cinnamomum camphora L.)

De novo comparative transcriptome analysis provides new insights into sucrose induced somatic embryogenesis in camphor tree (Cinnamomum camphora L.)

Temporary immersion systems (RITA®) for the improvement of cork oak somatic embryogenic culture proliferation and somatic embryo production

Plant regeneration of Picea asperata Mast. by somatic embryogenesis

Somatic embryogenesis in Alnus glutinosa (L.) Gaertn

Somatic embryogenesis and plant regeneration in Pterocarpus marsupium Roxb.

Micropropagation of camphor tree (Cinnamomum camphora)

Histocytological Study of Somatic Embryogenesis in the Tree Cinnamomum camphora L. (Lauraceae)

Effects of ozone exposure on sub-tropical evergreen Cinnamomum camphora seedlings grown in different nitrogen loads

Enhancement of somatic embryogenesis in camphor tree (Cinnamomum camphora L.): osmotic stress and other factors affecting somatic embryo formation on hormone-free medium

Similar Documents

Cyclic secondary somatic embryogenesis and efficient plant regeneration in camphor tree (Cinnamomum camphora L.)

De novo comparative transcriptome analysis provides new insights into sucrose induced somatic embryogenesis in camphor tree (Cinnamomum camphora L.)

Temporary immersion systems (RITA®) for the improvement of cork oak somatic embryogenic culture proliferation and somatic embryo production

Plant regeneration of Picea asperata Mast. by somatic embryogenesis

Somatic embryogenesis in Alnus glutinosa (L.) Gaertn

Somatic embryogenesis and plant regeneration in Pterocarpus marsupium Roxb.

Micropropagation of camphor tree (Cinnamomum camphora)

Histocytological Study of Somatic Embryogenesis in the Tree Cinnamomum camphora L. (Lauraceae)

Effects of ozone exposure on sub-tropical evergreen Cinnamomum camphora seedlings grown in different nitrogen loads

Enhancement of somatic embryogenesis in camphor tree (Cinnamomum camphora L.): osmotic stress and other factors affecting somatic embryo formation on hormone-free medium