NDLI: Auxin-mediated expression of a GH3 gene in relation to ontogenic state in Chestnut

Content Provider	Springer Nature Link
Author	Vielba, Jesus M. Varas, Elena Rico, Saleta Covelo, Purificación Sánchez, Conchi
Copyright Year	2016
Abstract	The CsGH3 - 1 gene was isolated from chestnut microshoots. Expression analysis during induction of adventitious roots indicates an alteration in shoot auxin homeostasis during maturation that negatively affects adventitious rooting. A new auxin inducible gene isolated from chestnut microshoots was found to encode a protein belonging to group II of the Gretchen Hagen 3 (GH3) family. The gene was, therefore, named CsGH3-1. Predicted protein sequence analysis revealed the presence of conserved domains involved in the conjugation of amino acids to indole-3-acetic acid (IAA). Modelling of the protein and molecular docking of IAA, indole-3-butyric-acid (IBA), 1-naphthaleneacetic acid (NAA), and benzothiazole-2-oxyacetic acid (BTOA) into the active site of CsGH3-1 indicated a high and similar binding affinity for the four substrates. Expression analysis by qPCR indicated that CsGH3-1 is regulated by wounding, darkness, and auxin in chestnut microshoots, in an ontogenetic-dependent manner. Under IBA treatment, upregulation of CsGH3-1 was higher in mature than in juvenile shoots and was negatively correlated with the ability of microshoots to form roots. High levels of auxin-induced expression of CsGH3-1 were detected in mature shoots 24 h after the IBA treatment, whereas transcript levels decreased in rooting-competent shoots with cell-type-specific expression. CsGH3-1 transcripts were specifically localized in cells involved in the initiation of adventitious roots only in rooting-competent shoots at the time when cells switch their fate to root initial cells. Thus, these data show a correlation between the specific localization of transcripts and rooting competence, and they also suggest a role for CsGH3-1 in regulating auxin homeostasis during the onset of adventitious root formation.
Starting Page	2237
Ending Page	2252
Page Count	16
File Format	PDF
ISSN	09311890
Journal	Trees
Volume Number	30
Issue Number	6
e-ISSN	14322285
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-08-05
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Adventitious roots Auxin Chestnut GH3 Molecular docking Ontogenic state Forestry Plant Sciences Agriculture Plant Anatomy/Development Plant Pathology Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Ecology Physiology Plant Science Forestry

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