NDLI: Verticillium lecanii spore production in solid-state and liquid-state fermentations

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Bioprocess Engineering : Volume 22

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Verticillium lecanii spore production in solid-state and liquid-state fermentations

Content Provider	Springer Nature Link
Author	Feng, K. C. Liu, B. L. Tzeng, Y. M.
Copyright Year	2000
Abstract	Verticillium lecanii has been recognized as an entomopathogen with high potential in biological control of pests. Two types of cultivation methods, the solid-state fermentation (SSF) and the liquid-state fermentation (LSF), were examined for V. lecanii. In SSF, the substrate types including rice, rice bran, rice husk, and the mixtures of these components were tested. The results showed that both cooked rice with appropriate water addition and rice bran gave significantly higher spore production of 1.5 × 109 spores/g substrate and 1.4 × 109 spores/g substrate, respectively. In LSF, SMAY liquid medium was used as a base, and the effects of environmental conditions on the spore production of V. lecanii were investigated. From the time course study, on the 9th day the spore yield reached 1.2 × 109 spores/ml of broth at 24 °C, 150 rpm for this strain. A series of medium volumes in the shaker-flask have been tested for the requirement of aeration. The largest surface aeration test, one tenth of the medium volume in the shaker-flask for cultivation, gave the highest spore count. The optimal pH value was tested and the initial pH 5 in the SMAY medium produced a high spore density. Finally, V. lecanii spores from SSF and LSF were different in size, shape, and size distribution; while mean spore length from SSF was 6.1 μm, and mean spore length from LSF was 5.0 μm.
Starting Page	25
Ending Page	29
Page Count	5
File Format	PDF
ISSN	0178515X
Journal	Bioprocess Engineering
Volume Number	23
Issue Number	1
e-ISSN	16157605
Language	English
Publisher	Springer-Verlag
Publisher Date	2000-07-27
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology Biotechnology

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