NDLI: Optimizing immobilization of avidin on surface-modified magnetic nanoparticles: characterization and application of protein-immobilized nanoparticles

Content Provider	Springer Nature Link
Author	Yang, Tao Sun, Shuguo Ma, Meihu Lin, Qinlu Zhang, Lin Li, Yan Luo, Feijun
Copyright Year	2015
Abstract	A simple optimization method of immobilization of avidin on magnetic nanoparticles (MNPs)’ surface was proposed in this study. The avidin-immobilized MNPs were then developed and used to immobilize a model enzyme [Horseradish peroxidase (HRP)]. The loading capacity (LC) and activity of avidin-immobilized MNPs were optimized through selecting the most appropriate nanoparticle’s size and shape, glutaraldehyde concentration, cross-linking reaction time, ultrasonic processing time, and initial concentration of avidin. The LC under optimized conditions was 63.37 ± 1.29 mg avidin/g MNPs, and the immobilized protein was still able to maintain its high biological activity of 10.86 ± 0.13 U/mg (biotin-binding activity of nature avidin was 14.1 U/mg) and better thermal stability compared to free avidin. A highly reusable, stable, and easily recovered immobilized HRP was obtained using MNPs as carriers. The immobilized HRP was reused repeatedly more than 9 times and retained more than 65 % of its original activity.
Starting Page	2023
Ending Page	2034
Page Count	12
File Format	PDF
ISSN	16157591
Journal	Bioprocess Engineering
Volume Number	38
Issue Number	10
e-ISSN	16157605
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-07-30
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Immobilization strategy Avidin Magnetic nanoparticles Biological activity Horseradish peroxidase Biotechnology Industrial and Production Engineering Environmental Engineering/Biotechnology Industrial Chemistry/Chemical Engineering Food Science
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Biotechnology

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