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Optimization of fibrolytic enzyme production by Aspergillus japonicus C03 with potential application in ruminant feed and their effects on tropical forages hydrolysis

Overcoming the production limitations of Photorhabdus temperata ssp. temperata strain K122 bioinsecticides in low-cost medium

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Overcoming the production limitations of Photorhabdus temperata ssp. temperata strain K122 bioinsecticides in low-cost medium

Content Provider	Springer Nature Link
Author	Jallouli, Wafa Jaoua, Samir Zouari, Nabil
Copyright Year	2011
Abstract	For low-cost production of Photorhabdus temperata ssp. temperata strain K122 bioinsecticide, a cheap complex medium was optimized. Diluted seawater was used as the source of micronutrients, especially sodium chloride, involved in the improvement of cell density, culturability and oral toxicity of the bacterium P. temperata against Ephestia kuehniella larvae. Thus, the new formulated medium was composed only of 10 g/l of soya bean meal, used as the carbon and nitrogen main source, mixed in sevenfold diluted seawater. At such conditions, several limitations of P. temperata bioinsecticide productions were shown to be overcome. The appearance of variants small colony polymorphism was completely avoided. Thus, the strain K122 was maintained at the primary form even after prolonged incubation. Moreover, the viable but nonculturable state was partially overcome, since the ability of P. temperata cells to form colonies on the solid medium was prolonged until 78 h of incubation. In addition, when cultured in the complex medium, P. temperata cells were produced at high cell density of 12 × 108 cells/ml and exhibited 81.48% improvement of oral toxicity compared to those produced in the optimized medium. With such medium, the large-scale bioinsecticides production into 3-l fully controlled fermenter improved the total cell counts, CFU counts and oral toxicity by 20, 5.81 and 16.73%, respectively. This should contribute to a significant reduction of production cost of highly potent P. temperata strain K122 cells, useful as a bioinsecticide.
Starting Page	1039
Ending Page	1047
Page Count	9
File Format	PDF
ISSN	16157591
Journal	Bioprocess Engineering
Volume Number	34
Issue Number	8
e-ISSN	16157605
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-06-09
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Photorhabdus temperata ssp. temperata strain K122 Bioinsecticide Variants small colony Viable but nonculturable state Low-cost medium Toxicity Industrial and Production Engineering Food Science Industrial Chemistry/Chemical Engineering Environmental Engineering/Biotechnology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Biotechnology

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