NDLI: Discontinuities in continuous cultures studied with a recombinant Saccharomyces cerevisiae and two computer models

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Bioprocess Engineering : Volume 17

Bioprocess Engineering : Volume 16

Discontinuities in continuous cultures studied with a recombinant Saccharomyces cerevisiae and two computer models

Content Provider	Springer Nature Link
Author	Ryszczuk, A. Grøn, S. Carlsen, M. Emborg, C.
Copyright Year	1998
Abstract	Chemostat cultures and other continuous cultures, where the feed to the bioreactor was divided in pulses while maintaining the overall dilutions rate by the increased flow in the pulses, were studied by cultivations with Saccharomyces cerevisiae JG176 and by simulations with two computer models, Yeast model SG176 and Yeast model MC176. All three systems gave interesting response surfaces. Deviations from an ideal chemostat may have significant effects on volumetric productivity, which for production of the recombinant protein, proteinase A by Saccharomyces cerevisiae JG176 was positive, while the productivity of biomass and ethanol decreased. In simulations with both models pulsing caused lower production of biomass and ethanol. In simulations with one of the models the effects of pulsing on productivity of a protein were also negative, whereas simulations with the other model suggested clear positive effects of pulsing on a production of a protein though with a somewhat different response surface than with the experiments with Saccharomyces cerevisiae JG176.
Starting Page	35
Ending Page	39
Page Count	5
File Format	PDF
ISSN	0178515X
Journal	Bioprocess Engineering
Volume Number	18
Issue Number	1
e-ISSN	16157605
Language	English
Publisher	Springer-Verlag
Publisher Date	1997-12-09
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology Biotechnology

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