NDLI: Parametric analysis of metabolic fluxes of α-amylase and protease-producing Bacillus subtilis

Content Provider	Springer Nature Link
Author	Skolpap, W. Nuchprayoon, S. Scharer, J. M. Moo Young, M.
Copyright Year	2007
Abstract	Parametric analysis was applied for a metabolic flux model for the fed-batch culture of Bacillus subtilis producing recombinant α-amylase and protease. The metabolic flux model was formulated as a linear programming problem consisting of 49 reactions (decision variables) and 50 metabolites (equality constraints). This study was aimed to determine the response of the metabolic fluxes and objective function value of minimizing the difference between ATP consumption and ATP production (ATP balance). With regard to intracellular metabolite accumulation, the objective function value was least sensitive to variation in succinate and most sensitive to variation in malate. Amongst the variations in the accumulation rates of extracellular metabolites, the objective function value was least sensitive to variation in glutamate and most sensitive to variation in starch hydrolysis and triglyceride synthesis. A 10% variation in metabolite accumulation rates caused a maximum of 13.8% variation (standard error = 3.8%) in the objective function value.
Starting Page	337
Ending Page	348
Page Count	12
File Format	PDF
ISSN	16157591
Journal	Bioprocess Engineering
Volume Number	30
Issue Number	5
e-ISSN	16157605
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-05-21
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Parametric analysis Flux balance analysis Bacillus subtilis Iterative linear programming Food Science Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution Waste Management/Waste Technology Industrial and Production Engineering Industrial Chemistry/Chemical Engineering Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Biotechnology

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