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| Content Provider | Springer Nature Link |
|---|---|
| Author | Choi, Seung Phill Bahn, Sang Hoon Sim, Sang Jun |
| Copyright Year | 2013 |
| Abstract | Botryococcus braunii, a green microalga, is known to produce plentiful liquid hydrocarbons as promising biodiesel resources. However, the hydrocarbon extraction methods that have so far achieved have several problems such as low efficiency and high cost. In our study, a solvent-spouted extraction process integrated with photo-bioculture was designed for simultaneous realization of hydrocarbon extraction and cell culture in two phases. The n-octane was selected as the best solvent among several solvents because its biocompatibility was highest for B. braunii. As a result, high level of biomass and hydrocarbon, 4.17 and 893.79 mg/L, respectively, was attained at 100 mL/min of solvent recycling rate through three times of processes for 66 days. Moreover, formation of cell clump was suppressed in solvent extraction, cells were regenerated after it, and thus cell viability was maintained even after repeated cycles of it. Finally, this solvent-spouted culture process required the smaller cost due to reuse of the less solvent and regenerated cells, compared with the other conventional methods. Accordingly, this technique would be applicable to exploit the continuous extraction of hydrocarbon from the algal biomass, especially for application on a large scale. |
| Starting Page | 1977 |
| Ending Page | 1985 |
| Page Count | 9 |
| File Format | |
| ISSN | 16157591 |
| Journal | Bioprocess Engineering |
| Volume Number | 36 |
| Issue Number | 12 |
| e-ISSN | 16157605 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2013-05-25 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Botryococcus braunii Cell regeneration Solvent extraction Hydrocarbon n-Octane Biotechnology Industrial and Production Engineering Environmental Engineering/Biotechnology Industrial Chemistry/Chemical Engineering Food Science |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Bioengineering Biotechnology |
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