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Enhanced biocatalytic production of l-cysteine by Pseudomonas sp. B-3 with in situ product removal using ion-exchange resin

Content Provider	Springer Nature Link
Author	Wang, Pu He, Jun Yao Yin, Jiang Feng
Copyright Year	2014
Abstract	Bioconversion of dl-2-amino-Δ2-thiazoline-4-carboxylic acid (dl-ATC) catalyzed by whole cells of Pseudomonas sp. was successfully applied for the production of l-cysteine. It was found, however, like most whole-cell biocatalytic processes, the accumulated l-cysteine produced obvious inhibition to the activity of biocatalyst and reduced the yield. To improve l-cysteine productivity, an anion exchange-based in situ product removal (ISPR) approach was developed. Several anion-exchange resins were tested to select a suitable adsorbent used in the bioconversion of dl-ATC for the in situ removal of l-cysteine. The strong basic anion-exchange resin 201 × 7 exhibited the highest adsorption capacity for l-cysteine and low adsorption for dl-ATC, which is a favorable option. With in situ addition of 60 g L−1 resin 201 × 7, the product inhibition can be reduced significantly and 200 mmol L−1 of dl-ATC was converted to l-cysteine with 90.4 % of yield and 28.6 mmol L−1 h−1 of volumetric productivity. Compared to the bioconversion without the addition of resin, the volumetric productivity of l-cysteine was improved by 2.27-fold using ISPR method.
Starting Page	421
Ending Page	428
Page Count	8
File Format	PDF
ISSN	16157591
Journal	Bioprocess Engineering
Volume Number	38
Issue Number	3
e-ISSN	16157605
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-09-10
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	dl-2-Amino-∆2-thiazoline-4-carboxylic acid l-Cysteine Pseudomonas sp. Bioconversion In situ product removal Anion-exchange resin Biotechnology Industrial and Production Engineering Environmental Engineering/Biotechnology Industrial Chemistry/Chemical Engineering Food Science
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Biotechnology

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Two-Stage Cultivation of Pseudomonas sp. F12 for the Production of Enzymes Converting dl-2-Amino-Δ$^{2}$-thiazoline-4-carboxylic Acid to l-Cysteine

Isolation and genetic improvement of Pseudomonas sp. strain HUT-78, capable of enzymatic production of L-cysteine from $DL-2-amino-Δ^{2}-thiazoline-4-carboxylic$ acid

Cloning, Expression, and Identification of Genes Involved in the Conversion of $DL-2-Amino-Δ^{2}-thiazoline-4-carboxylic$ Acid to L-Cysteine via S-Carbamyl-L-cysteine Pathway in Pseudomonas sp. TS1138

Medium Optimization for Enzymatic Production of L-Cysteine by Pseudomonas sp. Zjwp-14 Using Response Surface Methodology

Cloning, expression, characterization and application of atcA, atcB and atcC from Pseudomonas sp. for the production of l-cysteine

N-Carbamoyl-L-Cysteine as an Intermediate in the Bioconversion from D,L-2-Amino-Δ (2)-Thiazoline-4-Carboxylic Acid to L-Cysteine by Pseudomonas sp. ON-4a.

Enhanced dissolution of the substrate d,l-2-amino-Δ2-thiazoline-4-carboxylic acid and enzymatic production of l-cysteine at high concentrations

Two-stage cultivation of Pseudomonas sp. F12 for the production of enzymes converting DL-2-amino-Δ²-thiazoline-4-carboxylic acid to L-cysteine.

Enzymatic synthesis of L-tryptophan from D,L-2-amino-Δ2-thiazoline-4-carboxylic acid and indole by Pseudomonas sp. TS1138 L-2-amino-Δ2-thiazoline-4-carboxylic acid hydrolase, S-carbamyl-L-cysteine amidohydrolase and Escherichia coli L-tryptophanase

Enhanced biocatalytic production of l-cysteine by Pseudomonas sp. B-3 with in situ product removal using ion-exchange resin

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Two-Stage Cultivation of Pseudomonas sp. F12 for the Production of Enzymes Converting dl-2-Amino-Δ$^{2}$-thiazoline-4-carboxylic Acid to l-Cysteine

Isolation and genetic improvement of Pseudomonas sp. strain HUT-78, capable of enzymatic production of L-cysteine from $DL-2-amino-Δ^{2}-thiazoline-4-carboxylic$ acid

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Enhanced biocatalytic production of l-cysteine by Pseudomonas sp. B-3 with in situ product removal using ion-exchange resin