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Aerobic production of isoamyl acetate by overexpression of the yeast alcohol acetyl-transferases AFT1 and AFT2 in Escherichia coli and using low-cost fermentation ingredients

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Aerobic production of isoamyl acetate by overexpression of the yeast alcohol acetyl-transferases AFT1 and AFT2 in Escherichia coli and using low-cost fermentation ingredients

Content Provider	Springer Nature Link
Author	Singh, R. Vadlani, P. V. Harrison, M. L. Bennett, G. N. San, K. Y.
Copyright Year	2007
Abstract	Isoamyl acetate, produced via fermentation, is a natural flavor chemical with applications in the food industry. Two alcohol acetyltransferases from Saccharomyces cerevisiae (ATF1 and ATF2) can catalyze the esterification of isoamyl alcohol with acetyl coenzyme A. The respective genes were cloned and expressed in an appropriate ack-pta − strain of Escherichia coli. The engineered strains produce isoamyl acetate when isoamyl alcohol is added to the culture medium. Aerobic shake flask experiments examined isoamyl acetate production over various growth times, temperatures, and initial optical densities. The strain carrying the pBAD-ATF1 plasmid exhibited a high molar ester yield from glucose (1.13) after 48 h of aerobic growth at 25 °C. Low-cost media components, such as fusel oil, sorghum glucose and corn steep liquor, were found to give a high yield of isoamyl acetate. High-cell-density gave an increased isoamyl acetate yield of 0.18 g/g of glucose consumed.
Starting Page	299
Ending Page	306
Page Count	8
File Format	PDF
ISSN	16157591
Journal	Bioprocess Engineering
Volume Number	31
Issue Number	4
e-ISSN	16157605
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-09-21
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Escherichia coli Alcohol acetyltransferases Isoamyl acetate Fusel oil Sorghum Food Science Industrial Chemistry/Chemical Engineering Environmental Engineering/Biotechnology Industrial and Production Engineering Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Biotechnology

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Aerobic production of isoamyl acetate by overexpression of the yeast alcohol acetyl-transferases AFT1 and AFT2 in Escherichia coli and using low-cost fermentation ingredients

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