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| Content Provider | Springer Nature Link |
|---|---|
| Author | Jang, K. H. Seo, J. W. Song, K. B. Kim, C. H. Rhee, S. K. |
| Copyright Year | 1999 |
| Abstract | Secretion of levansucrase from Zymomonas mobilis in Escherichiacoli by glycine supplement was investigated. A significant amount of levansucrase (about 25% of total activity) was found in intact whole-cells. Cell fractionation experiments showed that levansucrase was found both in the periplasmic space and in the cytoplasmic fraction of E. coli. None or only trace amounts of levansucrase was detected in the extracellular culture broth at 24 h of cultivation and it accrued with the increasing concentration of glycine in the culture medium and duration of the culture period. Optimal glycine concentration for the maximum secretion of levansucrase was in the range of 0.8–1%, in which approximately 20–50% of levansucrase was released into the extracellular fraction at 24 h of cultivation, although glycine retarded the bacterial growth. |
| Starting Page | 453 |
| Ending Page | 458 |
| Page Count | 6 |
| File Format | |
| ISSN | 0178515X |
| Journal | Bioprocess Engineering |
| Volume Number | 21 |
| Issue Number | 5 |
| e-ISSN | 16157605 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 1999-11-04 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Content Type | Text |
| Resource Type | Article |
| Subject | Applied Microbiology and Biotechnology Biotechnology |
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