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| Content Provider | Springer Nature Link |
|---|---|
| Author | Mafra, Agnes Cristina Oliveira Furlan, Felipe Fernando Badi, Alberto Colli Tardioli, Paulo Waldir |
| Copyright Year | 2014 |
| Abstract | Sucrose from sugarcane is produced in abundance in Brazil, which provides an opportunity to manufacture other high-value products. Gluconic acid (GA) can be produced by multi-enzyme conversion of sucrose using the enzymes invertase, glucose oxidase, and catalase. In this process, one of the byproducts is fructose, which has many commercial applications. This work concerns the batch mode production of GA in an airlift reactor fed with sucrose as substrate. Evaluation was made of the influence of temperature and pH, as well as the thermal stability of the enzymes. Operational conditions of 40 °C and pH 6.0 were selected, based on the enzymatic activity profiles and the thermal stabilities. Under these conditions, the experimental data could be accurately described by kinetic models. The maximum yield of GA was achieved within 3.8 h, with total conversion of sucrose and glucose and a volumetric productivity of around 7.0 g L−1 h−1. |
| Starting Page | 671 |
| Ending Page | 680 |
| Page Count | 10 |
| File Format | |
| ISSN | 16157591 |
| Journal | Bioprocess Engineering |
| Volume Number | 38 |
| Issue Number | 4 |
| e-ISSN | 16157605 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2014-10-19 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Gluconic acid Fructose Sucrose Airlift bioreactors Batch processing Multi-enzyme bioconversion Biotechnology Industrial and Production Engineering Environmental Engineering/Biotechnology Industrial Chemistry/Chemical Engineering Food Science |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Bioengineering Biotechnology |
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