NDLI: Carboxymethylcellulose–gelatin–superoxidase dismutase electrode for amperometric superoxide radical sensing

Content Provider	Springer Nature Link
Author	Kocabay, Ozge Emregul, Emel Aras, Sümer Emregul, Kaan Cebesoy
Copyright Year	2012
Abstract	A novel, highly sensitive superoxide dismutase biosensor for the direct and simultaneous determination of superoxide radicals was developed by immobilization of superoxide dismutase within carboxymethylcellulose–gelatin on a Pt electrode surface. The parameters affecting the performance of the biosensor were investigated. The response of the CMC–G–SOD biosensor was proportional to O 2 ·− concentration and the detection limit was 1.25 × 10−3 mM with a correlation coefficient of 0.9994. The developed biosensor exhibited high analytical performance with wider linear range, high sensitivity and low response time. The biosensor retained 89.8% of its sensitivity after use for 80 days. The support system enhanced the immobilization of superoxide dismutase and promoted the electron transfer of superoxide dismutase minimizing its fouling effect. The biosensor was quite effective not only in detecting O 2 ·− , but also in determining the antioxidant properties of acetylsalicylic acid-based drugs and the anti-radical activity of healthy and cancerous human brain tissues.
Starting Page	923
Ending Page	930
Page Count	8
File Format	PDF
ISSN	16157591
Journal	Bioprocess Engineering
Volume Number	35
Issue Number	6
e-ISSN	16157605
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-01-18
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Carboxymethylcellulose–gelatin Superoxide dismutase Biosensor Superoxide anions Scavengers Industrial and Production Engineering Biotechnology Industrial Chemistry/Chemical Engineering Environmental Engineering/Biotechnology Food Science
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Biotechnology

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