NDLI: Gene expression, cellular localization, and enzymatic activity of diacylglycerol kinase isozymes in rat ovary and placenta

Content Provider	Springer Nature Link
Author	Toya, Mayumi Hozumi, Yasukazu Ito, Tsukasa Takeda, Morihiko Sakane, Fumio Kah, Hideo Saito, Hidekazu Hiroi, Masahiko Kurachi, Hirohisa Kondo, Hisatake Goto, Kaoru
Copyright Year	2005
Abstract	Female reproductive organs show remarkable cyclic changes in morphology and function in response to a combination of hormones. Evidence has accumulated suggesting that phosphoinositide turnover and the consequent diacylglycerol (DG) protein kinase C (PKC) pathway are intimately involved in these mechanisms. The present study has been performed to investigate the gene expression, cellular localization, and enzymatic activity of the DG kinase (DGK) isozymes that control the DG-PKC pathway. Gene expression for DGKα, -ε, -ζ, and -ι was detected in the ovary and placenta. Intense expression signals for DGKζ and -α were observed in the theca cells and moderate signals in the interstitium and corpora lutea of the ovary. On the other hand, signals for DGKε were seen more intensely in granulosa cells. In the placenta, signals for DGKα and -ι were observed in the junctional zone, whereas those for DGKζ were detected in the labyrinthine zone. At higher magnification, the signals for DGKα were mainly discerned in giant cytotrophoblasts, and those for DGKι were found in small cytotrophoblasts of the junctional zone. DGKζ signals were observed in all cellular components of the labyrinthine zone, including mesenchyme, trabecular trophoblasts, and cytotrophoblasts. DGKε signals were detected in the junctional zone on day 13 and 15 of pregnancy and were diffusely distributed both in the labyrinthine and junctional zones at later stages. The present study reveals distinct patterns of mRNA localization for DGK isozymes in the rat ovary and placenta, suggesting that each isozyme plays a unique role in distinct cell types in these organs.
Starting Page	525
Ending Page	533
Page Count	9
File Format	PDF
ISSN	0302766X
Journal	Cell and Tissue Research
Volume Number	320
Issue Number	3
e-ISSN	14320878
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-04-26
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Diacylglycerol kinase Diacylglycerol protein kinase C pathway Ovary Placenta Subcellular localization Rat (Wistar, female)
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Pathology and Forensic Medicine

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