NDLI: Dynamics of apoptosis in the ovarian follicle cells during the late stages of Drosophila oogenesis

Content Provider	Springer Nature Link
Author	Nezis, Ioannis P. Stravopodis, Dimitrios J. Papassideri, Issidora Robert Nicoud, Michel Margaritis, Lukas H.
Copyright Year	2002
Abstract	In the present study, we demonstrate the apoptotic events of the ovarian follicle cells during the late stages of oogenesis in Drosophila melanogaster. Follicle cell morphology appears normal from stage 10 up to stage 14, exhibiting a euchromatic nucleus and a well-organized cytoplasm. First signs of apoptosis appear at the anterior pole of the egg chamber at stage 14A. They are characterized by loss of microvilli at the apical cell membrane, alterations in nuclear morphology, such as chromatin condensation and convolution of the nuclear membrane, and also by condensation and vacuolization of the cytoplasm. During the following stage 14B, the follicle cell nuclei contain fragmented DNA as is demonstrated by acridine orange staining and TUNEL (TdT-mediated dUTP nick end-labeling) assay. Finally, the apoptotic follicle cells seem to detach from the eggshell when the mature egg chamber exits the ovariole. The detached follicle cells exhibit condensed nuclear chromatin, a disorganized cytoplasm with crowded organelles and are surrounded by epithelial cells. The above results seem to be associated with the abundant phagocytosis that we observed at the entry of the lateral oviducts, where the epithelial cells contain apoptotic cell bodies. Additionally, we tested the effect of etoposide treatment in the follicular epithelium and found that it induces apoptosis in a stage- and site-specific manner. These observations suggest a possible method of absorption of the apoptotic follicle cells that prevents the blockage of the ovarioles and helps the regular production of mature eggs.
Starting Page	401
Ending Page	409
Page Count	9
File Format	PDF
ISSN	0302766X
Journal	Cell and Tissue Research
Volume Number	307
Issue Number	3
e-ISSN	14320878
Language	English
Publisher	Springer-Verlag
Publisher Date	2002-02-02
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Pathology and Forensic Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Mechanisms of programmed cell death during oogenesis in Drosophila virilis

Tracking nucleolar dynamics with GFP-Nopp140 during Drosophila oogenesis and embryogenesis

Apoptosis of nurse cells at the late stages of oogenesis of Drosophila melanogaster

Counting in oogenesis

Expression of caspase 3 in ovarian follicle cells of the lizard Podarcis sicula

Exclusion of dysfunctional mitochondria from Balbiani body during early oogenesis of Thermobia

Inhibitor of apoptosis proteins and ovarian dysfunction in galactosemic rats

Identification of cells migrating from the thecal layer of ovarian follicles

Role of Notch pathway in terminal follicle cell differentiation during Drosophila oogenesis

Dynamics of apoptosis in the ovarian follicle cells during the late stages of Drosophila oogenesis

Similar Documents

Mechanisms of programmed cell death during oogenesis in Drosophila virilis

Tracking nucleolar dynamics with GFP-Nopp140 during Drosophila oogenesis and embryogenesis

Apoptosis of nurse cells at the late stages of oogenesis of Drosophila melanogaster

Counting in oogenesis

Expression of caspase 3 in ovarian follicle cells of the lizard Podarcis sicula

Exclusion of dysfunctional mitochondria from Balbiani body during early oogenesis of Thermobia

Inhibitor of apoptosis proteins and ovarian dysfunction in galactosemic rats

Identification of cells migrating from the thecal layer of ovarian follicles

Role of Notch pathway in terminal follicle cell differentiation during Drosophila oogenesis

Dynamics of apoptosis in the ovarian follicle cells during the late stages of Drosophila oogenesis