NDLI: Characterization of an extremely motile cellular network in the rotifer Asplanchna spp. : Structure, kinetics, and the cytoskeleton

Content Provider	Springer Nature Link
Author	Baumann, Otto Arlt, Kathleen Römmling, Katja Goller, Helmut Walz, Bernd
Copyright Year	1999
Abstract	The pseudocoelomic body cavity of the rotifer Asplanchna spp. contains free cells that form a highly dynamic, three-dimensional polygonal network of filopodia. Using video-enhanced differential interference contrast microscopy, we have qualitatively and quantitatively characterized the motion types involved with network motility: (1) filopodial junctions are displaced laterally at 10.52±0.46 µm/s; (2) free-ending filopodia form and extend at rates of 8.77±0.40 µm/s, until they retract again at 7.23±0.87 µm/s; (3) filopodial strands fuse either laterally or tip to the lateral side. The combination of these motion types results in enlargements, diminutions, and extinctions of filopodial polygons, and in the formation of new polygons. Moreover, there is intense and fast (5.11±0.28 µm/s) particle transport within the filopodial strands. The organization of the cytoskeleton in filopodia was examined by electron microscopy and by labeling with fluorescent-tagged phalloidin. Filopodia contain several microtubules that are often organized in a bundle. Moreover, F-actin is present within the filopodia. To characterize which of these cytoskeletal systems is involved with cell and organelle motility, we have examined cell dynamics after incubations with colchicine or cytochalasin D. The results of these pharmacological experiments provide evidence that microtubules are required for both cell and organelle motility, but that actin filaments contribute to these phenomena and are required for the structural maintenance of slender filopodia.
Starting Page	159
Ending Page	172
Page Count	14
File Format	PDF
ISSN	0302766X
Journal	Cell and Tissue Research
Volume Number	299
Issue Number	1
e-ISSN	14320878
Language	English
Publisher	Springer-Verlag
Publisher Date	1999-11-30
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Book Review
Subject	Cell Biology Histology Pathology and Forensic Medicine

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