NDLI: IGF-I binding and receptor signal transduction in primary cell culture of muscle cells of gilthead sea bream: changes throughout in vitro development

Content Provider	Springer Nature Link
Author	Montserrat, N. Sánchez Gurmaches, J. García de la Serrana, D. Navarro, M. I. Gutiérrez, J.
Copyright Year	2007
Abstract	We examined the possibility of culturing muscle cells of gilthead sea bream in vitro and assessed variations in insulin-like growth factor-I (IGF-I) binding during myocyte development. The viability of the cell culture was determined by fluorescence-activated cell-sorting analysis, which showed that the percentage of dead cells decreased with cell differentiation. The intracellular reduction of MTT into formazan pigment was preferentially carried out as cells differentiated (from day 4) indicating an increase in metabolic activity. IGF-I-binding assays demonstrated that the number of receptors increased from 190 ± 0.09 fmol/mg protein in myocytes at day 5 to 360 ± 0.09 fmol/mg protein in myotubes at day12. The affinity of IGF-I receptors did not change significantly during cell development (from 0.89 ± 0.09 to 0.98 ± 0.09 nM). The activation of various kinase (ERK 1/2 MAPK and Akt/PKB) proteins by IGFs and insulin was studied by means of Western blot analysis. Levels of MAPK-P increased after IGF and insulin treatment during the first stages of cell culture, with a low response being observed at day 15, whereas IGFs displayed a stimulatory effect on Akt-P throughout the cell culture period, even on day 15. This study thus shows that (1) gilthead sea bream myocytes can be cultured, (2) they express functional IGF-I receptors that increase in number as they differentiate in vitro; (3) IGF signalling transduction through IGF-I receptors stimulates the MAPK and Akt pathways, depending on the development stage of the muscle cell culture.
Starting Page	503
Ending Page	513
Page Count	11
File Format	PDF
ISSN	0302766X
Journal	Cell and Tissue Research
Volume Number	330
Issue Number	3
e-ISSN	14320878
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-10-17
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Muscle cells Insulin Insulin-like growth factors Insulin-like growth factor-I receptor Signal transduction Gilthead sea bream Sparus aurata (Teleostei) Molecular Medicine Proteomics Human Genetics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Pathology and Forensic Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Seasonal change in circulating growth hormone (GH) hepatic GH-binding and plasma insulin-like growth factor 1 immunoreactivity in a marine fish gilthead sea bream Sparus aurata

Isolation of Staphylococcus hominis from Cultured Gilthead Sea Bream (Sparus aurata L.) in Antalya Bay, Turkey

Isolation of Staphylococcus hominis from Cultured Gilthead Sea Bream (Sparus aurata L.) in Antalya Bay, Turkey

Growth hormone expression in ontogenic development in gilthead sea bream

Insulin-like growth factor 1 (IGF-1) regulates prolactin, growth hormone, and IGF-1 receptor expression in the pituitary gland of the gilthead sea bream Sparus aurata

Characterization data of gilthead sea bream (Sparus aurata) IGF-I receptors (IGF-IRa/Rb)

Growth hormone as an in vitro phagocyte-activating factor in the gilthead sea bream (Sparus aurata)

Cellular localisation of insulin-like growth factor binding protein-2 (IGFBP-2) during development of the marine fish, Sparus aurata

Metabolic Effects of Insulin and IGFs on Gilthead Sea Bream (Sparus aurata) Muscle Cells

IGF-I binding and receptor signal transduction in primary cell culture of muscle cells of gilthead sea bream: changes throughout in vitro development

Similar Documents

Seasonal change in circulating growth hormone (GH) hepatic GH-binding and plasma insulin-like growth factor 1 immunoreactivity in a marine fish gilthead sea bream Sparus aurata

Isolation of Staphylococcus hominis from Cultured Gilthead Sea Bream (Sparus aurata L.) in Antalya Bay, Turkey

Isolation of Staphylococcus hominis from Cultured Gilthead Sea Bream (Sparus aurata L.) in Antalya Bay, Turkey

Growth hormone expression in ontogenic development in gilthead sea bream

Insulin-like growth factor 1 (IGF-1) regulates prolactin, growth hormone, and IGF-1 receptor expression in the pituitary gland of the gilthead sea bream Sparus aurata

Characterization data of gilthead sea bream (Sparus aurata) IGF-I receptors (IGF-IRa/Rb)

Growth hormone as an in vitro phagocyte-activating factor in the gilthead sea bream (Sparus aurata)

Cellular localisation of insulin-like growth factor binding protein-2 (IGFBP-2) during development of the marine fish, Sparus aurata

Metabolic Effects of Insulin and IGFs on Gilthead Sea Bream (Sparus aurata) Muscle Cells

IGF-I binding and receptor signal transduction in primary cell culture of muscle cells of gilthead sea bream: changes throughout in vitro development