NDLI: Immunolocalization of a Gαq protein to the chemosensory organs of Dipolydora quadrilobata (Polychaeta: Spionidae)

Content Provider	Springer Nature Link
Author	Tsie, Marlene S. Rawson, Paul D. Lindsay, Sara M.
Copyright Year	2008
Abstract	Chemoreception in marine invertebrates mediates a variety of ecologically important behaviors including defense, reproduction, larval settlement and recruitment, and feeding. The sensory pathways that regulate deposit-feeding activity by polychaetes living in sedimentary habitats are of particular interest because such feeding has profound effects on the physical and chemical properties of the habitat. Nevertheless, little is known concerning the molecular mechanisms of chemical signal transduction associated with deposit feeding and other behaviors in polychaetes. Chemosensory-based feeding behaviors are typically regulated by G-protein-coupled signal transduction pathways. However, the presence and role of such pathways have not been demonstrated in marine polychaetes. Methodologies involving degenerate primer-based reverse transcription with the polymerase chain reaction and rapid amplification of cDNA ends were used to identify and characterize a Gαq subunit expressed in the feeding palps of the spionid polychaete Dipolydora quadrilobata. The D. quadrilobata Gαq protein had high sequence similarity with previously reported Gαq subunits from both invertebrate and vertebrate taxa. Immunhistochemistry and immunocytochemistry were used with confocal laser scanning microscopy and transmission electron microscopy to visualize the distribution of a Gαq antibody in whole worms and in cilia of the feeding palps. Gαq immunoreactivity was concentrated in the nuchal organs, food-groove cilia, and lateral/abfrontal cilia of the feeding palps. Because these structures are known to be involved in chemoreception, we propose that Gαq isolated from D. quadrilobata is a key component of chemosensory signal transduction pathways in this species.
Starting Page	469
Ending Page	480
Page Count	12
File Format	PDF
ISSN	0302766X
Journal	Cell and Tissue Research
Volume Number	333
Issue Number	3
e-ISSN	14320878
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-07-05
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chemoreception G-protein Immunohistochemistry Dipolydora quadrilobata (Annelida, Polychaeta) Molecular Medicine Proteomics Human Genetics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Pathology and Forensic Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Immunofluorescence analysis of the internal brain anatomy of Nereis diversicolor (Polychaeta, Annelida)

Lipoprotein mediated lipid uptake in oocytes of polychaetes (Annelida)

Immunolocalization of α1A-adrenoceptors in rat and human epididymis

Tail regenerative capacity and iNOS immunolocalization in Xenopus laevis tadpoles

Secretory cells of the airway express molecules of the chemoreceptive cascade

Cholinergic epithelial cell with chemosensory traits in murine thymic medulla

Immunolocalization of aquaporin-4 in the brain, kidney, skeletal muscle, and gastro-intestinal tract of chicken

Endothelial cell biology and pathology

Expression and localization of GPR91 and GPR99 in murine organs

Immunolocalization of a Gαq protein to the chemosensory organs of Dipolydora quadrilobata (Polychaeta: Spionidae)

Similar Documents

Immunofluorescence analysis of the internal brain anatomy of Nereis diversicolor (Polychaeta, Annelida)

Lipoprotein mediated lipid uptake in oocytes of polychaetes (Annelida)

Immunolocalization of α1A-adrenoceptors in rat and human epididymis

Tail regenerative capacity and iNOS immunolocalization in Xenopus laevis tadpoles

Secretory cells of the airway express molecules of the chemoreceptive cascade

Cholinergic epithelial cell with chemosensory traits in murine thymic medulla

Immunolocalization of aquaporin-4 in the brain, kidney, skeletal muscle, and gastro-intestinal tract of chicken

Endothelial cell biology and pathology

Expression and localization of GPR91 and GPR99 in murine organs

Immunolocalization of a Gαq protein to the chemosensory organs of Dipolydora quadrilobata (Polychaeta: Spionidae)