NDLI: Vascular endothelial growth factor-A165 (VEGF-A165) stimulates the in vitro development and oocyte competence of goat preantral follicles

Content Provider	Springer Nature Link
Author	Araújo, V. R. Silva, G. M. Duarte, A. B. G. Magalhães, D. M. Almeida, A. P. Gonçalves, R. F. B. Bru, J. B. Silva, T. F. P. Campello, C. C. Rodrigues, A. P. R. Figueiredo, J. R.
Copyright Year	2011
Abstract	The aim of this study was to evaluate the effect of vascular endothelial growth factor-A165 (VEGF-A165) on the in vitro development of goat secondary preantral follicles. Preantral follicles (≥150 μm in diameter) were isolated from the ovaries of adult mixed-breed goats and individually cultured for 18 days in αMEM in the absence (control) or presence of VEGF-A165 at concentrations of 10 ng/ml (VEGF10) and 100 ng/ml (VEGF100). Analyses of follicular survival, diameter, antrum formation and rate of daily growth were performed every 6 days. At the end of the culture period, morphologically normal oocytes (≥110 μm in diameter) were taken for in vitro maturation (IVM). The results demonstrated that all follicles presented oocytes and granulosa cells that were morphologically normal and after labeling with calcein-AM, high rates of oocyte viability were observed in all treatments. The follicular diameter and the growth rate achieved in the presence of VEGF10 were higher than those of the control. Both treatments with VEGF-A165 showed higher rates of oocyte recovery for IVM when compared with the control. Moreover, only the addition of VEGF-A165 permitted oocytes grown in vitro to reach metaphase II. Thus, the addition of VEGF-A165 to the culture medium improves the development of goat preantral follicles cultured in vitro, allowing the production of mature oocytes.
Starting Page	273
Ending Page	281
Page Count	9
File Format	PDF
ISSN	0302766X
Journal	Cell and Tissue Research
Volume Number	346
Issue Number	2
e-ISSN	14320878
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-10-11
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	In vitro growth Maturation VEGF-A165 Ovarian follicles Growth factors Goat Molecular Medicine Proteomics Human Genetics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Pathology and Forensic Medicine

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