NDLI: Environmental parameters influence non-viral transfection of human mesenchymal stem cells for tissue engineering applications

Content Provider	Springer Nature Link
Author	King, William J. Kouris, Nicholas A. Choi, Siyoung Ogle, Brenda M. Murphy, William L.
Copyright Year	2012
Abstract	Non-viral transfection is a promising technique that could be used to increase the therapeutic potential of stem cells. The purpose of this study was to explore practical culture parameters of relevance in potential human mesenchymal stem cell (hMSC) clinical and tissue engineering applications, including type of polycationic transfection reagent, N/P ratio and dose of polycation/pDNA polyplexes, cell passage number, cell density and cell proliferation. The non-viral transfection efficiency was significantly influenced by N/P ratio, polyplex dose, cell density and cell passage number. hMSC culture conditions that inhibited cell division also decreased transfection efficiency, suggesting that strategies to promote hMSC proliferation may be useful to enhance transfection efficiency in future tissue engineering studies. Non-viral transfection treatments influenced hMSC phenotype, including the expression level of the hMSC marker CD105 and the ability of hMSCs to differentiate down the osteogenic and adipogenic lineages. The parameters found here to promote hMSC transfection efficiency, minimize toxicity and influence hMSC phenotype may be instructive in future non-viral transfection studies and tissue engineering applications.
Starting Page	689
Ending Page	699
Page Count	11
File Format	PDF
ISSN	0302766X
Journal	Cell and Tissue Research
Volume Number	347
Issue Number	3
e-ISSN	14320878
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-01-26
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Adult stem cells Multipotency Bioprocessing Toxicity Polyethyleneimine Human Genetics Molecular Medicine Proteomics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Pathology and Forensic Medicine

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