NDLI: High resolution neurochemical gold staining method for myelin in peripheral and central nervous system at the light- and electron-microscopic level

Content Provider	Springer Nature Link
Author	Savaskan, Nic E. Weinmann, Oliver Heimrich, Bernd Eyupoglu, Ilker Y.
Copyright Year	2009
Abstract	Myelin is a multilamellar membrane structure primarily composed of lipids and myelin proteins essential for proper neuronal function. Since myelin is a target structure involved in many pathophysiological conditions such as metabolic, viral, and autoimmune diseases and genetic myelin disorders, a reliable myelin detection technique is required that is equally suitable for light- and electron-microscopic analysis. Here, we report that single myelinated fibers are specifically stained by the gold phosphate complex, Black gold, which stains myelin in the brain, spinal cord, and peripheral nerve fibers in a reliable manner. Electron-microscopic and morphometric analyses have revealed that gold particles are equally distributed in the inner, compact, and outer myelin layers. In contrast to Luxol fast blue, the gold dye stains proteinase-sensitive myelin structures, indicating its selective labeling of myelin-specific proteins. Aiming at defining the target of gold staining, we performed staining in several mouse myelin mutants. Gold complex distribution and myelin staining in MBP−/−/shiverer mouse mutants was comparable with that seen in wild-type mice but revealed a more clustered Black gold distribution. This gold staining method thus provides a sensitive and specific high-resolution marker for both central and peripheral myelin sheaths; it also allows the quantitative analysis of myelinated fibers at the light- and electron-microscopic level suitable for investigations of myelin and axonal disorders.
Starting Page	213
Ending Page	221
Page Count	9
File Format	PDF
ISSN	0302766X
Journal	Cell and Tissue Research
Volume Number	337
Issue Number	2
e-ISSN	14320878
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-06-10
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Neurohistopathological staining technique Myelin Demyelinating disease Central nervous system Peripheral nervous system Reticulon-4/Nogo A Myelin associated glycoprotein (MAG) Mouse (shiverer mutant) Molecular Medicine Proteomics Human Genetics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Pathology and Forensic Medicine

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The Formation Patterns of Central Myelin Sheaths in the Myelin Deficient Mutant Shiverer Mouse

Shiverer and Normal Peripheral Myelin Compared: Basic Protein Localization, Membrane Interactions, and Lipid Composition

Developmental Alterations in Molecular Weights of Proteins in the Human Central Nervous System That React With Antibodies Against Myelin-Associated Glycoprotein

The myelinassociated glycoprotein is enriched in multivesicular bodies and periaxonal membranes of actively myelinating oligodendrocytes (1989)

2′-3′-cyclic nucleotide 3′-phosphohydrolase activity in the central nervous system of a myelin deficient mutant (Shiverer)

Myelin-associated glycoprotein (MAG) in the CNS of adult shiverer (Shi/Shi) mice.

A Nogo signal coordinates the perfect match between myelin and axons.

Immunoreactive Myelin Basic Proteins Are Not Detected When Shiverer Mutant Schwann Cells and Fibroblasts Are Co-cultured with Normal Neurons

Myelin-Associated Glycoprotein: Electron Microscopic Immunocytochemical Localization in Compact Developing and Adult Central Nervous System Myelin

High resolution neurochemical gold staining method for myelin in peripheral and central nervous system at the light- and electron-microscopic level

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Immunoreactive Myelin Basic Proteins Are Not Detected When Shiverer Mutant Schwann Cells and Fibroblasts Are Co-cultured with Normal Neurons

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