NDLI: Integrin expression and integrin-mediated adhesion in vitro of human multipotent stromal cells (MSCs) to endothelial cells from various blood vessels

Content Provider	Springer Nature Link
Author	Semon, Julie A. Nagy, Lauren H. Llamas, Claire B. Tucker, H. Alan Lee, Ryang Hwa Prockop, Darwin J.
Copyright Year	2010
Abstract	Multipotent mesenchymal stromal cells (MSCs) home to damaged tissue by processes partly regulated by integrins. Integrin subunits expressed by MSCs were identified by flow cytometry (FC), immunocytochemistry (IC), and a panel of integrin-binding antibodies. In subconfluent cultures, over 80% of MSCs expressed integrin subunits β1, β2, and α3, 20%–55% expressed α1, α2, α4, α5, α6, and αV, and about 10% expressed β3 when assayed by FC. None of the cells expressed significant levels of 13 other integrins as assayed by FC, but seven of the 13 integrins were detected by IC: β5, α7, α8, α9, α11, αX, and αD. Expression of some integrins changed with MSC confluency: integrins β3, α1, α3, α5, and αV increased, and α6 decreased. Furthermore, α4 was the only integrin to vary among preparations of MSCs from different donors. The results resolved some discrepancies in the literature concerning integrin expression by MSCs. We also investigated the role of specific integrins in MSC adhesion to endothelial cells (ECs) from the pulmonary artery (HPAEC), cardiac-derived microvasculature (HMVEC-C), and umbilical veins (HUVEC). In experiments with blocking antibodies to beta integrins, anti-β5 reduced MSC adhesion to all types of ECs, anti-β1 to both HUVEC and HPAEC, anti-β3 to HUVEC, and anti-β2 to HMVEC-C. With blocking antibodies to alpha integrins, anti-αX reduced adhesion to HPAEC and HMVEC-C, anti-αV to HPAEC, and both anti-α7 and anti-αD to HMVEC-C. Thus, MSCs use diverse integrins to adhere to EC from various blood vessels in vitro.
Starting Page	147
Ending Page	158
Page Count	12
File Format	PDF
ISSN	0302766X
Journal	Cell and Tissue Research
Volume Number	341
Issue Number	1
e-ISSN	14320878
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-06-20
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Multipotent mesenchymal stromal cells Adhesion Integrin Trafficking Homing Human Molecular Medicine Proteomics Human Genetics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Pathology and Forensic Medicine

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