NDLI: Vesicular glutamate transporter 2 (VGLUT2) is co-stored with PACAP in projections from the rat melanopsin-containing retinal ganglion cells

Content Provider	Springer Nature Link
Author	Engelund, Anna Fahrenkrug, Jan Harrison, Adrian Hannibal, Jens
Copyright Year	2010
Abstract	The retinal ganglion cell layer of the eye comprises a subtype of cells characterized by their intrinsic photosensitivity and expression of melanopsin (ipRGCs). These cells regulate a variety of non-image-forming (NIF) functions such as light entrainment of circadian rhythms, acute suppression of locomotor activity (masking), and pupillary light reflex. Two neurotransmitters have been identified in ipRGCs, glutamate and pituitary adenylate cyclase-activating polypeptide (PACAP). To date, little is known about their release and interplay. Here, we describe the presence and co-localization of vesicular glutamate transporter 2 (VGLUT2; a marker of glutamate signaling) and PACAP in ipRGCs and their projections in the brain. Nine adult male Wistar rats were assigned to one of three groups; anterograde tracing (n = 3), eye enucleation (n = 3), and untreated (n = 3). Under anaesthesia, rats were transcardially perfusion-fixated, after which the brains and eyes were removed for double immunohistochemical staining using a polyclonal anti-VGLUT2 antibody and a mouse monoclonal anti-PACAP antibody. Results revealed that VGLUT2- and PACAP-immunoreactivity (-ir) were present in ipRGCs and co-localized in their projections in the suprachiasmatic nucleus, the intergeniculate leaflet, and the olivary pretectal nucleus. We conclude that there is evidence to support the use of glutamate and PACAP as neurotransmitters in NIF photoperception by rat ipRGCs, and that these neurotransmitters are co-stored and probably released from the same nerve terminals. Furthermore, we conclude that VGLUT2 is the preferred subtype of vesicular transporter used by these cells.
Starting Page	243
Ending Page	255
Page Count	13
File Format	PDF
ISSN	0302766X
Journal	Cell and Tissue Research
Volume Number	340
Issue Number	2
e-ISSN	14320878
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-03-26
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	PACAP VGLUT2 Melanopsin Retina RHT Rat (Wistar) Molecular Medicine Proteomics Human Genetics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Pathology and Forensic Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Target areas innervated by PACAP-immunoreactive retinal ganglion cells

Melanopsin is expressed in pacap-containing retinal ganglion cells of the human retinohypothalamic tract.

Vesicular glutamate transporter 2-immunoreactive afferent nerve terminals in the carotid body of the rat

Melanopsin is expressed in PACAP-containing retinal ganglion cells of the human retinohypothalamic tract.

Impaired Circadian Photosensitivity in Mice Lacking Glutamate Transmission from Retinal Melanopsin Cells

Co-localization of Gamma-Aminobutyric Acid and Glutamate in Neurons of the Spider Central Nervous System

Synaptic contact between melanopsin-containing retinal ganglion cells and rod bipolar cells.

Protective effects of the neuropeptide PACAP in diabetic retinopathy

Changes in morphology of retinal ganglion cells with eccentricity in retinal degeneration

Vesicular glutamate transporter 2 (VGLUT2) is co-stored with PACAP in projections from the rat melanopsin-containing retinal ganglion cells

Similar Documents

Target areas innervated by PACAP-immunoreactive retinal ganglion cells

Melanopsin is expressed in pacap-containing retinal ganglion cells of the human retinohypothalamic tract.

Vesicular glutamate transporter 2-immunoreactive afferent nerve terminals in the carotid body of the rat

Melanopsin is expressed in PACAP-containing retinal ganglion cells of the human retinohypothalamic tract.

Impaired Circadian Photosensitivity in Mice Lacking Glutamate Transmission from Retinal Melanopsin Cells

Co-localization of Gamma-Aminobutyric Acid and Glutamate in Neurons of the Spider Central Nervous System

Synaptic contact between melanopsin-containing retinal ganglion cells and rod bipolar cells.

Protective effects of the neuropeptide PACAP in diabetic retinopathy

Changes in morphology of retinal ganglion cells with eccentricity in retinal degeneration

Vesicular glutamate transporter 2 (VGLUT2) is co-stored with PACAP in projections from the rat melanopsin-containing retinal ganglion cells