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| Content Provider | Springer Nature Link |
|---|---|
| Author | He, Qing Lian Zhu, Ya Zhen Zheng, Guang Juan Shi, Ling Chun Hu, Shao Wei Li, Chu Tian |
| Copyright Year | 2012 |
| Abstract | Cell block sections serve as an important diagnostic annex for cytological smears, liquid-based SurePath cytology and the Liquid-based Thin-prep Cytology Test (TCT). A variety of methods for the preparation of cell blocks are described in the literature and the techniques in cell blocks are in continuous improvement. A new technique for making cell blocks was introduced in the present study. We first used pregelatinized starch as the frame for the cell block, which is a really simple and economic method, because it can be carried out at room temperature without additional special instruments. We have performed hematoxylin and eosin (HE) staining, immunohistochemistry analysis and fluorescence in situ hybridization (FISH) in the cell block sections in 122 cytological specimens. The results demonstrated in this article show that pregelatinized starch is a useful frame for cell blocks. The pregelatinized starch can effectively collect even a few cells with powerful adhesiveness. Therefore, this new technique for making cell blocks is especially useful for cytologic samples with low cellularity, such as cerebrospinal fluid specimens. |
| Starting Page | 395 |
| Ending Page | 400 |
| Page Count | 6 |
| File Format | |
| ISSN | 0302766X |
| Journal | Cell and Tissue Research |
| Volume Number | 350 |
| Issue Number | 2 |
| e-ISSN | 14320878 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2012-08-16 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Body fluid Cell block Pregelatinized starch Cytology Molecular Medicine Proteomics Human Genetics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Histology Pathology and Forensic Medicine |
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