NDLI: Autocrine growth promotion by multiple hematopoietic growth factors in the established renal cell carcinoma line KU-19

Content Provider	Springer Nature Link
Author	Tachibana, Masaaki Miyakawa, Ayako Nakashima, Jun Murai, Masaru Nakamura, Kayoko Kubo, Atsushi Hata, Jun ichi
Copyright Year	2000
Abstract	Increasing evidence suggests that paraneoplastic syndrome may be mediated by tumor-related cytokine release, although the specific factors involved remain to be clearly defined. The cancer cells used in the present study were obtained from a 67-year-old man with metastatic renal cell carcinoma in the subcutaneous space who demonstrated marked leukocytosis (37,800/mm3). The primary tumor of the kidney was pathologically diagnosed as renal cell carcinoma consistent with the sarcomatoid type. On microscopic observation, the cultured cells exhibited an epithelial appearance with vacuole formation in their cytoplasm. Ultrastructural observations revealed relatively marked microvilli and a tight junction. Significant amounts of GM-CSF, G-CSF, IL-6, and IL-8 concentrations in the culture media were identified by an enzyme-linked immunosorbent assay. Reverse transcriptase polymerase chain reaction (RT-PCR) significantly exhibited marker protein m-RNA expression in cancer cells. In addition, GM-CSF receptor and IL-6 receptor mRNA expression was also demonstrated by RT-PCR. The administration of both IL-6 and GM-CSF induced cell-proliferation activities estimated by both [3H]-thymidine and bromodeoxyuridine labeling. Anti-IL-6 antibody and anti-GM-CSF antibody neutralized the enhanced proliferative activities generated by these cytokines. Our findings indicate that the established renal cancer cell line can be demonstrated by both the production of multiple cytokines and by their promotion of autocrine growth. These cells are thus considered to be useful as an effective model for multipotent differentiated renal cell carcinoma, as well as for studying the mechanisms of action of autocrine growth.
Starting Page	353
Ending Page	367
Page Count	15
File Format	PDF
ISSN	0302766X
Journal	Cell and Tissue Research
Volume Number	301
Issue Number	3
e-ISSN	14320878
Language	English
Publisher	Springer-Verlag
Publisher Date	2000-07-12
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Pathology and Forensic Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Regulation of vascular development by fibroblast growth factors

Induction of cardiogenesis by Hensen's node and fibroblast growth factors

Endothelins – vasoactive peptides and growth factors

Immunolocalization of vascular endothelial growth factor in the GH3 cell line

Autocrine and paracrine interactions and neuroprotection in glaucoma

Differentiation of rat thymic myoid progenitor cell line established by coculture with human T-lymphotropic virus type-I-producing human T cells

Cellular swirls in fine needle aspirates of papillary renal cell carcinoma.

An in vivo model of prostate carcinoma growth and invasion in bone

Imprint cytology of oncocytic papillary renal cell carcinoma.

Autocrine growth promotion by multiple hematopoietic growth factors in the established renal cell carcinoma line KU-19–20

Similar Documents

Regulation of vascular development by fibroblast growth factors

Induction of cardiogenesis by Hensen's node and fibroblast growth factors

Endothelins – vasoactive peptides and growth factors

Immunolocalization of vascular endothelial growth factor in the GH3 cell line

Autocrine and paracrine interactions and neuroprotection in glaucoma

Differentiation of rat thymic myoid progenitor cell line established by coculture with human T-lymphotropic virus type-I-producing human T cells

Cellular swirls in fine needle aspirates of papillary renal cell carcinoma.

An in vivo model of prostate carcinoma growth and invasion in bone

Imprint cytology of oncocytic papillary renal cell carcinoma.

Autocrine growth promotion by multiple hematopoietic growth factors in the established renal cell carcinoma line KU-19–20