NDLI: Fibronectin and laminin induce expression of islet cell markers in hepatic oval cells in culture

Content Provider	Springer Nature Link
Author	Leite, Adriana Ribeiro Corrêa Giannella, Maria Lúcia Dagli, Maria Lúcia Zaidan Fortes, Maria Angela Zanela Vegas, Vanina Monique Tucci Giannella Neto, Daniel
Copyright Year	2006
Abstract	Hepatic oval cells (OC) are considered to be facultative liver stem cells and, because they may undergo differentiation into a variety of cell lineages, they might have the potential to be used in cellular therapy. Signals delivered by extracellular matrix (ECM) proteins take part in cellular differentiation in cooperation with signals from growth factors; indeed, some ECM proteins, such as laminin (LAM) and fibronectin (FN), have been shown to contribute to β-cell differentiation and islet development, respectively. Since no previous studies have investigated the effect of ECM proteins on the expression of islet cell markers by cultured OC, the purpose of the present study was to evaluate whether FN and LAM modulate the expression of genes related to the endocrine pancreas in these liver cells. OC proliferation was induced in Wistar rats by prolonged treatment with 2-acetoaminofluorene/allyl alcohol and confirmed by reverse transcription/polymerase chain reaction and hepatic immunocytochemical and histopathological analyses. OC isolation was performed by Ficoll gradient and magnetic-activated cell sorting. OC were cultured for 1 and 2 months under several conditions with specific growth factors, over a FN or LAM substrate or in high glucose, nicotinamide and fetal calf serum. OC cultured on FN substrate expressed Pdx-1, Pax-6, insulin 2 and glucagon. LAM also induced the expression of Pdx-1, insulin 1 and insulin 2, glucagon, somatostatin and GLUT-2. Our results suggest that these ECM proteins can be used in protocols of OC transdifferentiation aimed at reducing the period necessary for complete transdifferentiation.
Starting Page	529
Ending Page	537
Page Count	9
File Format	PDF
ISSN	0302766X
Journal	Cell and Tissue Research
Volume Number	327
Issue Number	3
e-ISSN	14320878
Language	English
Publisher	Springer-Verlag
Publisher Date	2006-12-06
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Hepatic oval cell Differentiation Fibronectin Laminin Endocrine pancreas Rat (Wistar, male) Molecular Medicine Proteomics Human Genetics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Histology Pathology and Forensic Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Different ghrelin localisation in adult human and rat endocrine pancreas

Localization of myosin-Va in subpopulations of cells in rat endocrine organs

In vitro long-term development of cultured inner ear stem cells of newborn rat

Differentiation of human adipose-derived stem cells towards cardiomyocytes is facilitated by laminin

Immunohistochemical and gene expression analysis of stem-cell-associated markers in rat dental pulp

Activation of stem cells in hepatic diseases

Rnh1 promotes differentiation and myelination via RhoA in oligodendrocytes

Enhanced expression of fibroblast growth factor 2 in bone marrow cells and its potential role in the differentiation of hepatic epithelial stem-like cells into the hepatocyte lineage

Immunolocalization of α1A-adrenoceptors in rat and human epididymis

Fibronectin and laminin induce expression of islet cell markers in hepatic oval cells in culture

Similar Documents

Different ghrelin localisation in adult human and rat endocrine pancreas

Localization of myosin-Va in subpopulations of cells in rat endocrine organs

In vitro long-term development of cultured inner ear stem cells of newborn rat

Differentiation of human adipose-derived stem cells towards cardiomyocytes is facilitated by laminin

Immunohistochemical and gene expression analysis of stem-cell-associated markers in rat dental pulp

Activation of stem cells in hepatic diseases

Rnh1 promotes differentiation and myelination via RhoA in oligodendrocytes

Enhanced expression of fibroblast growth factor 2 in bone marrow cells and its potential role in the differentiation of hepatic epithelial stem-like cells into the hepatocyte lineage

Immunolocalization of α1A-adrenoceptors in rat and human epididymis

Fibronectin and laminin induce expression of islet cell markers in hepatic oval cells in culture