NDLI: Functional features of the C-terminal region of yeast ribosomal protein L5

Content Provider	Springer Nature Link
Author	Moradi, Hossein Simoff, Ivailo Bartish, Galyna Nygård, Odd
Copyright Year	2008
Abstract	The aim of this study was to analyze the functional importance of the C-terminus of the essential yeast ribosomal protein L5 (YrpL5). Previous studies have indicated that the C-terminal region of YrpL5 forms an α-helix with a positively charged surface that is involved in protein–5S rRNA interaction. Formation of an YrpL5·5S rRNA complex is a prerequisite for nuclear import of YrpL5. Here we have tested the importance of the α-helix and the positively charged surface for YrpL5 function in Saccharomyces cerevisiae using site directed mutagenesis in combination with functional complementation. Alterations in the sequence forming the putative α-helix affected the functional capacity of YrpL5. However, the effect did not correlate with a decreased ability of the protein to bind to 5S rRNA as all rpL5 mutants tested were imported to the nucleus whether or not the α-helix or the positively charged surface were intact. The alterations introduced in the C-terminal sequence affected the growth rate of cells expressing mutant but functional forms of YrpL5. The reduced growth rate was correlated with a reduced ribosomal content per cell indicating that the alterations introduced in the C-terminus interfered with ribosome assembly.
Starting Page	337
Ending Page	350
Page Count	14
File Format	PDF
ISSN	16174615
Journal	Molecular and General Genetics MGG
Volume Number	280
Issue Number	4
e-ISSN	16174623
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-08-27
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Functional complementation Mutation analysis Ribosomal protein L5 S. cerevisiae Animal Genetics and Genomics Plant Genetics & Genomics Microbial Genetics and Genomics Biochemistry Cell Biology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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