NDLI: A virulent parent with probiotic progeny: comparative genomics of Escherichia coli strains CFT073, Nissle 1917 and ABU 83972

Content Provider	Springer Nature Link
Author	Vejborg, Rebecca Munk Friis, Carsten Hancock, Viktoria Schembri, Mark A. Klemm, Per
Copyright Year	2010
Abstract	Escherichia coli is a highly versatile species encompassing a diverse spectrum of strains, i.e. from highly virulent isolates causing serious infectious diseases to commensals and probiotic strains. Although much is known about bacterial pathogenicity in E. coli, the understanding of which genetic determinants differentiates a virulent from an avirulent strain still remains limited. In this study we designed a new comparative genomic hybridization microarray based on 31 sequenced E. coli strains and used it to compare two E. coli strains used as prophylactic agents (i.e. Nissle 1917 and 83972) with the highly virulent uropathogen CFT073. Only relatively minor genetic variations were found between the isolates, suggesting that the three strains may have originated from the same virulent ancestral parent. Interestingly, Nissle 1917 (a gut commensal strain) was more similar to CFT073 with respect to genotype and phenotype than 83972 (an asymptomatic bacteriuria strain). The study indicates that genetic variations (e.g. mutations) and expression differences, rather than genomic content per se, contribute to the divergence in disease-causing ability between these strains. This has implications for the use of virulence factors in epidemiological research, and emphasizes the need for more comparative genomic studies of closely related strains to compare their virulence potential.
Starting Page	469
Ending Page	484
Page Count	16
File Format	PDF
ISSN	16174615
Journal	Molecular and General Genetics MGG
Volume Number	283
Issue Number	5
e-ISSN	16174623
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-03-31
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	CFT073 Nissle 1917 ABU 83972 Comparative genomic hybridization analysis Escherichia coli Urinary tract infection Animal Genetics and Genomics Microbial Genetics and Genomics Cell Biology Plant Genetics & Genomics Biochemistry
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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