NDLI: Isolation and characterization of novel F-plasmid sopB mutants defective in gene silencing

Content Provider	Springer Nature Link
Author	Kubo, Y. Kim, S. K. Hanai, R.
Copyright Year	2001
Abstract	The product of the sopB gene on the Escherichia coli F-plasmid has been shown to silence genes in the vicinity of its binding region, sopC, when overexpressed. We searched for mutants defective in SopB-dependent silencing by screening for a plasmid incompatibility phenotype, in order to examine the relationship between gene silencing and the intracellular localization of SopB, as revealed by a green fluorescent protein (GFP)-SopB fusion. Nine new mutants were isolated. One of them, in which leucine 92 is replaced by proline, was completely compatible with a sopC-carrying plasmid and was defective in other silencing activities. When expressed as a GFP fusion protein, the L92P mutant was found to be uniformly distributed in the cell. This implies a link between silencing and SopB localization, supporting the view that a high local concentration of SopB drives non-specific DNA binding in segments of the plasmid adjacent to sopC. Despite the lack of apparent localization of GFP fluorescence, the mutant protein, like the wild-type SopB, was found mostly in the inner membrane fraction, indicating that the association with the inner membrane was retained.
Starting Page	806
Ending Page	812
Page Count	7
File Format	PDF
ISSN	16174615
Journal	Molecular and General Genetics MGG
Volume Number	266
Issue Number	5
e-ISSN	16174623
Language	English
Publisher	Springer-Verlag
Publisher Date	2001-10-20
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Defective plasmid partition in ftsH mutants of Escherichia coli

Characterization of Chlamydomonas mutants defective in the H subunit of Mg-chelatase

Purification and characterization of SopA and SopB proteins essential for F plasmid partitioning.

Isolation and characterization of novel mutants of Arabidopsis thaliana defective in flower development

Molecular characterization and isolation of the F/f gene for femaleness in cucumber (Cucumis sativus L.)

Isolation and characterization of novel plasmid-encoded umuC mutants.

Isolation of a new set of Aspergillus nidulans mutants defective in nuclear migration

Isolation and molecular characterization of the carboxy-terminal pdr3 mutants in Saccharomyces cerevisiae

Characterization and global gene expression of F− phenocopies during Escherichia coli biofilm formation

Isolation and characterization of novel F-plasmid sopB mutants defective in gene silencing

Similar Documents

Defective plasmid partition in ftsH mutants of Escherichia coli

Characterization of Chlamydomonas mutants defective in the H subunit of Mg-chelatase

Purification and characterization of SopA and SopB proteins essential for F plasmid partitioning.

Isolation and characterization of novel mutants of Arabidopsis thaliana defective in flower development

Molecular characterization and isolation of the F/f gene for femaleness in cucumber (Cucumis sativus L.)

Isolation and characterization of novel plasmid-encoded umuC mutants.

Isolation of a new set of Aspergillus nidulans mutants defective in nuclear migration

Isolation and molecular characterization of the carboxy-terminal pdr3 mutants in Saccharomyces cerevisiae

Characterization and global gene expression of F− phenocopies during Escherichia coli biofilm formation

Isolation and characterization of novel F-plasmid sopB mutants defective in gene silencing