NDLI: Contribution of 5′- and 3′-untranslated regions of plastid mRNAs to the expression of Chlamydomonas reinhardtii chloroplast genes

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Contribution of 5′- and 3′-untranslated regions of plastid mRNAs to the expression of Chlamydomonas reinhardtii chloroplast genes

Content Provider	Springer Nature Link
Author	Barnes, Dwight Franklin, Scott Schultz, Jason Henry, Ryan Brown, Emma Coragliotti, Anna Mayfield, Stephen P.
Copyright Year	2005
Abstract	Expression of chloroplast genes is primarily regulated posttranscriptionally, and a number of RNA elements, found in either the 5′- or 3′-untranslated regions (UTRs) of plastid mRNAs, that impact gene expression have been identified. Complex regulatory and feedback mechanisms influence both translation and protein accumulation, making assignment of roles for specific RNA elements difficult. To identify specific contributions made by various UTRs on translation of plastid mRNAs, we used a heterologous gfp reporter gene that is fused combinatorially to chloroplast 5′- and 3′-UTRs. In general, the 5′-UTR, including the promoter, of the plastid atpA and psbD genes produced the highest levels of chimeric mRNA and protein accumulation, while the 5′-UTR of the rbcL and psbA genes produced less mRNA and protein. Varying the 3′-UTR had little impact on mRNA and protein accumulation, as long as a 3′-UTR was present. Overall, accumulation of chimeric mRNAs was proportional to protein accumulation, with a few notable exceptions. Light-regulated translation continues to operate in chimeric mRNAs containing the 5′-UTR of either the psbA or psbD mRNAs, despite translation of these two chimeric mRNAs at very different efficiencies, suggesting that translational efficiency and light-regulated translation are separate events. Translation of some chimeric mRNAs was much more efficient than others, suggesting that interactions between the untranslated and coding sequences can dramatically impact translational efficiency.
Starting Page	625
Ending Page	636
Page Count	12
File Format	PDF
ISSN	16174615
Journal	Molecular and General Genetics MGG
Volume Number	274
Issue Number	6
e-ISSN	16174623
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-10-18
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	5′-UTR 3′-UTR Chloroplast Gene expression Animal Genetics and Genomics Plant Genetics & Genomics Microbial Genetics and Genomics Biochemistry Cell Biology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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Translational Regulation of Chloroplast Genes PROTEINS BINDING TO THE 5′-UNTRANSLATED REGIONS OF CHLOROPLAST mRNAs IN CHLAMYDOMONAS REINHARDTII

Relationship between mRNA levels and protein accumulation in a chloroplast promoter-mutant of Chlamydomonas reinhardtii

Insensitivity of chloroplast gene expression to DNA methylation

Specific sequence elements in the 5′ untranslated regions of rbcL and atpB gene mRNAs stabilize transcripts in the chloroplast of Chlamydomonas reinhardtii

Generation of an oligonucleotide array for analysis of gene expression in Chlamydomonas reinhardtii

Identification of target mRNAs for the clock-controlled RNA-binding protein Chlamy 1 from Chlamydomonas reinhardtii

The barley plastome mutant CL2 affects expression of nuclear and chloroplast housekeeping genes in a cell-age dependent manner

Functional analysis of 5′ untranslated region of a TIR-NBS-encoding gene from triploid white poplar

Specific sequence elements in the 5' untranslated regions of rbcL and atpB gene mRNas stabilize transcripts in the chloroplast of Chlamydomonas reinhardtii.

Contribution of 5′- and 3′-untranslated regions of plastid mRNAs to the expression of Chlamydomonas reinhardtii chloroplast genes

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Translational Regulation of Chloroplast Genes PROTEINS BINDING TO THE 5′-UNTRANSLATED REGIONS OF CHLOROPLAST mRNAs IN CHLAMYDOMONAS REINHARDTII

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Specific sequence elements in the 5' untranslated regions of rbcL and atpB gene mRNas stabilize transcripts in the chloroplast of Chlamydomonas reinhardtii.

Contribution of 5′- and 3′-untranslated regions of plastid mRNAs to the expression of Chlamydomonas reinhardtii chloroplast genes