NDLI: Comparison of skeletal muscle miRNA and mRNA profiles among three pig breeds

Content Provider	Springer Nature Link
Author	Hou, Xinhua Yang, Yalan Zhu, Shiyun Hua, Chaoju Zhou, Rong Mu, Yulian Tang, Zhonglin Li, Kui
Copyright Year	2015
Abstract	The pig is an important source of animal protein, and is also an ideal model for human disease. There are significant differences in growth rate, muscle mass, and meat quality between different breeds. To understand the molecular mechanisms underlying porcine skeletal muscle phenotypes, we performed mRNA and miRNA profiling of muscle from three different breeds of pig, Landrace (lean-type), Tongcheng (obese-type), and Wuzhishan (mini-type) by Solexa sequencing. Forty-three genes and 106 miRNAs were differentially expressed between Landrace and Tongcheng pigs, 92 genes and 151 miRNAs were differentially expressed between Tongcheng and Wuzhishan pigs, and 145 genes and 156 miRNAs were differential expressed between Landrace and Wuzhishan pigs. Gene ontology analysis suggested that genes differentially expressed between Landrace and Tongcheng pigs were mainly involved in the biological processes of oxidative stress and muscle organ development. Meanwhile, for Tongcheng vs Wuzhishan and Landrace vs Wuzhishan pigs, the differentially expressed genes were involved in fatty acid metabolism, oxidative stress, muscle contraction, and muscle organ development, processes that are closely related to meat quality. To investigate the molecular mechanisms underlying meat quality diversity based on differentially expressed genes and miRNAs, interaction networks were constructed, according to target prediction results and integration analysis of up-regulated genes with down-regulated miRNAs or down-regulated genes with up-regulated miRNAs. Our findings identify candidate genes and miRNAs associated with muscle development and indicate their potential roles in muscle phenotype variance between different pig breeds. These results serve as a foundation for further studies on muscle development and molecular breeding.
Starting Page	559
Ending Page	573
Page Count	15
File Format	PDF
ISSN	16174615
Journal	Molecular and General Genetics MGG
Volume Number	291
Issue Number	2
e-ISSN	16174623
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-10-12
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	miRNAs Transcriptome Solexa sequencing Pig Skeletal muscles Cell Biology Biochemistry Microbial Genetics and Genomics Plant Genetics & Genomics Animal Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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