NDLI: Identification of growth phenotype-related genes in Aspergillus oryzae by heterologous macroarray and suppression subtractive hybridization

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Identification of growth phenotype-related genes in Aspergillus oryzae by heterologous macroarray and suppression subtractive hybridization

Content Provider	Springer Nature Link
Author	Biesebeke, R. Levin, A. Sagt, C. Bartels, J. Goosen, T. Ram, A. Hondel, C. Punt, P.
Copyright Year	2005
Abstract	Aspergillus oryzae requires polarized growth for colonization of solid substrates, and this growth phenotype differs from that seen in liquid medium. Various experimental approaches were used to identify genes that are differentially expressed when A. oryzae is grown on wheat kernels and in a wheat-based liquid medium. Hybridization of A. oryzae RNAs to a macroarray bearing cDNAs isolated from a library representing at least 16% of the total number of A. niger genes identified 14 differentially expressed cDNA clones, showing that heterologous macroarray analysis with an A. niger cDNA library can be used to identify regulated gene transcripts in the related species A. oryzae. Moreover, Northern analysis with a selection of eight probes for A. niger genes encoding proteins involved in morphological development and cell wall biosynthesis identified five more differentially expressed genes. A suppression subtractive hybridization procedure revealed another 12 differentially expressed genes. The results presented show that, of the 29 identified genes which are expressed at higher levels during growth on wheat kernels, six encode proteins that are functionally related to polarized growth, four encode products known to be involved in morphogenesis, three code for proteins related to cell wall composition, and nine of the cDNA clones encode novel proteins. These findings pinpoint genes associated with the changes in cellular morphogenesis seen in A. oryzae grown on wheat kernels as opposed to wheat-based liquid medium.
Starting Page	33
Ending Page	42
Page Count	10
File Format	PDF
ISSN	16174615
Journal	Molecular and General Genetics MGG
Volume Number	273
Issue Number	1
e-ISSN	16174623
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-01-28
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Aspergillus Macroarray hybridization Polarized growth Morphology Heterologous hybridization
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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