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| Content Provider | Springer Nature Link |
|---|---|
| Author | Jiang, Liang Huang, Chunlin Sun, Qiang Guo, Huizhen Peng, Zhengwen Dang, Yinghui Liu, Weiqiang Xing, Dongxu Xu, Guowen Zhao, Ping Xia, Qingyou |
| Copyright Year | 2014 |
| Abstract | Bombyx mori and mulberry constitute a model of insect–host plant interactions. Urease hydrolyzes urea to ammonia and is important for the nitrogen metabolism of silkworms because ammonia is assimilated into silk protein. Silkworms do not synthesize urease and acquire it from mulberry leaves. We synthesized the artificial DNA sequence ure-as using the codon bias of B. mori to encode the signal peptide and mulberry urease protein. A transgenic vector that overexpresses ure-as under control of the silkworm midgut-specific P2 promoter was constructed. Transgenic silkworms were created via embryo microinjection. RT-PCR results showed that urease was expressed during the larval stage and qPCR revealed the expression only in the midgut of transgenic lines. Urea concentration in the midgut and hemolymph of transgenic silkworms was significantly lower than in a nontransgenic line when silkworms were fed an artificial diet. Analysis of the daily body weight and food conversion efficiency of the fourth and fifth instar larvae and economic characteristics indicated no differences between transgenic silkworms and the nontransgenic line. These results suggested that overexpression of host plant urease promoted nitrogen metabolism in silkworms. |
| Starting Page | 1117 |
| Ending Page | 1123 |
| Page Count | 7 |
| File Format | |
| ISSN | 16174615 |
| Journal | Molecular and General Genetics MGG |
| Volume Number | 290 |
| Issue Number | 3 |
| e-ISSN | 16174623 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2014-12-31 |
| Publisher Place | Berlin/Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Insect–plant interactions Mulberry Overexpression Silkworm Transgenic Urease Cell Biology Biochemistry Microbial Genetics and Genomics Plant Genetics & Genomics Animal Genetics and Genomics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Medicine Molecular Biology |
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