NDLI: The rice OsLOL2 gene encodes a zinc finger protein involved in rice growth and disease resistance

Content Provider	Springer Nature Link
Author	Xu, Chunxiao He, Chaozu
Copyright Year	2007
Abstract	Arabidopsis LSD1-related proteins that contain LSD1-like zinc finger domains have been identified to be involved in disease resistance and programmed cell death. To investigate the potential role of LSD1-related gene in rice (Oryza sativa L.), we cloned an LSD1 ortholog, OsLOL2, from the rice cDNA plasmid library. The OsLOL2 gene is predicted to encode a polypeptide of 163 amino acids with two LSD1-like zinc finger domains with 74.5% identity to those of LSD1. Southern blot analysis indicated that OsLOL2 was a single-copy gene in the rice genome. Transgenic rice lines carrying the antisense strand of OsLOL2 with decreased expression of OsLOL2 had dwarf phenotypes, and the dwarfism could be restored by exogenous GA3 treatment, suggesting that the dwarfism was the result of a deficiency in bioactive gibberellin (GA). In agreement with this possibility, the content of endogenous bioactive GA1 decreased in the antisense transgenic lines. Expression of OsKS1, one of the genes encoding for GA biosynthetic enzymes, was suppressed in the antisense transgenic lines. Sense transgenic lines with increased expression of OsLOL2 were more resistant to rice bacterial blight, while antisense transgenic lines were less resistant to rice bacterial blight. The OsLOL2-GFP (green fluorescence protein) fusion protein was localized in the nucleus of cells of transgenic BY2 tobacco (Nicotiana tabacum L.). These data suggest that OsLOL2 is involved in rice growth and disease resistance.
Starting Page	85
Ending Page	94
Page Count	10
File Format	PDF
ISSN	16174615
Journal	Molecular and General Genetics MGG
Volume Number	278
Issue Number	1
e-ISSN	16174623
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-04-03
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Disease resistance Dwarf OsLOL2 Rice Zinc finger protein Microbial Genetics and Genomics Biochemistry Cell Biology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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