Please wait, while we are loading the content...
Please wait, while we are loading the content...
| Content Provider | Springer Nature Link |
|---|---|
| Author | Goel, Apollina Vohra, Harpreet Varshney, Grish C. |
| Copyright Year | 1999 |
| Abstract | Surface antigens on Leishmania promastigotes and infected macrophages are obvious targets in immunoprophylaxis for leishmanial infection. We have recently demonstrated that the polyclonal antiserum and monoclonal antibodies generated by homologous immunizations with the crude membranes of parasite-infected cells react effectively with the `neo-antigenic' determinants on the infected cell surface. In the present study, we investigated the utility of such polyclonal antisera for identifying `minor' surface components of promastigotes. The reactivity of anti-Leishmania donovani-(strain RMRI68) infected macrophage membrane (anti-IMm) antiserum was compared with that of anti-promastigote (anti-Pr) antiserum towards the infected macrophage surface and promastigotes of three Indian strains of L. donovani, RMRI68, AG83 and DD8. While anti-Pr antiserum showed no reactivity with the infected macrophage surface but reacted strongly with air dried and live promastigotes of all three strains, anti-IMm antiserum reacted with the infected cell surface and, interestingly, specifically recognized live promastigotes of the strain used for infection, i.e., strain RMRI68. The reactivity patterns of the two antisera with the immunodominant components of the L. donovani promastigote surface, i.e., purified LPG-KMP11 complex and gp63 molecules, indicated that unlike anti-Pr antiserum, the specificities in anti-IMm antiserum were mainly directed towards molecules other than the LPG-KMP11 complex and gp63. Antiserum generated in a similar fashion against the macrophage membrane of cells infected in vitro with strain AG83 also contained antibodies specific to strain AG83 promastigotes. The present approach may therefore greatly help in identifying specific antigen(s) important in clinical and epidemiological control of leishmaniasis. |
| Starting Page | 19 |
| Ending Page | 24 |
| Page Count | 6 |
| File Format | |
| ISSN | 09320113 |
| Journal | Parasitology Research |
| Volume Number | 85 |
| Issue Number | 1 |
| e-ISSN | 14321955 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 1999-01-01 |
| Publisher Place | Berlin/Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Content Type | Text |
| Resource Type | Article |
| Subject | Infectious Diseases Veterinary Parasitology Insect Science |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|