NDLI: In vitro antifilarial activity of glutathione S-transferase inhibitors

Content Provider	Springer Nature Link
Author	Srinivasan, Lakshmy Mathew, Nisha Muthuswamy, Kalyanasundaram
Copyright Year	2009
Abstract	Female adult bovine filarial worms Setaria digitata were extracted with phosphate-buffered saline (pH 7.4) and glutathione S-transferase (GST) activity and protein content were determined. The protein content, GST enzyme activity, and specific activity were 10.61 ± 3.41 mg ml−1, 0.09 ± 0.019 μmol min−1 ml−1, and 0.009 ± 0.002 μmol min−1 mg−1 protein, respectively. The GST inhibition studies were performed with and without the inhibitors resulted from earlier molecular docking studies viz., ethacrynic acid, plumbagin, and curcumin for which the IC50 values were 19.42, 51.41, and 114.86 μM, respectively. The in vitro macrofilaricidal activity of these molecules was studied by worm motility and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay at 24- and 48-h incubation. Plumbagin and ethacrynic acid showed 100% inhibition in worm motility at lower concentrations of 3.19 and 6.6 μM, respectively, at 48-h incubation while curcumin was effective at 54.29 μM. In MTT reduction assay, the ED50 values (50% inhibition in formazan formation) for plumbagin, ethacrynic acid, and curcumin at 48-h incubation were 1.20, 2.48, and 19.86 μM, respectively. MTT reduction assay showed that plumbagin was the most effective in killing the adult S. digitata worms followed by ethacrynic acid and curcumin. In conclusion, all the three molecules selected by molecular modeling and docking studies inhibited the GST enzyme isolated from S. digitata and exhibited macrofilaricidal activity in vitro.
Starting Page	1179
Ending Page	1182
Page Count	4
File Format	PDF
ISSN	09320113
Journal	Parasitology Research
Volume Number	105
Issue Number	4
e-ISSN	14321955
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-06-28
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Immunology Microbiology Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary Parasitology Insect Science

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