NDLI: The bacterial catalase from filarial DNA preparations derives from common pseudomonad contaminants and not from Wolbachia endosymbionts

Content Provider	Springer Nature Link
Author	Foster, Jeremy Baldo, Laura Blaxter, Mark Henkle Dührsen, Kimberly Whitton, Claire Slatko, Barton Bandi, Claudio
Copyright Year	2004
Abstract	Wolbachia are obligatory endosymbionts in many species of filarial nematodes. Certain bacterial molecules induce antibody responses in mammalian hosts infected with filariae, while others activate inflammatory responses that contribute to pathology. These findings, coupled with antibiotic studies demonstrating the dependence of filarial embryogenesis on the presence of Wolbachia, have intensified research on Wolbachia–nematode interactions, and the effects of Wolbachia molecules on the mammalian immune system. By amplification and sequencing of 16S rDNA and catalase sequences, we show that filarial DNA samples prepared from nematodes collected under typical conditions are frequently contaminated with Pseudomonas DNA. Analysis of a published DNA fragment containing a catalase attributed to the Wolbachia of Onchocerca volvulus showed it to be most like Pseudomonas, both in terms of sequence similarity and genomic organization. Additionally, there was no obvious catalase in either of two available Wolbachia genome sequences. Contamination of filarial DNA with bacterial sequences other than Wolbachia can complicate studies of the role of these symbionts in filarial biology.
Starting Page	141
Ending Page	146
Page Count	6
File Format	PDF
ISSN	09320113
Journal	Parasitology Research
Volume Number	94
Issue Number	2
e-ISSN	14321955
Language	English
Publisher	Springer-Verlag
Publisher Date	2004-08-19
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary Parasitology Insect Science

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Molecular identification of Wolbachia from the filarial nematode Mansonella ozzardi

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DNA extraction from Ascaris suum muscle tissue

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Biochemical studies on glutathione S-transferase from the bovine filarial worm Setaria digitata

Absence of Wolbachia endobacteria in the non-filariid nematodes Angiostrongylus cantonensis and A. costaricensis

Supergroup F Wolbachia bacteria parasitise lice (Insecta: Phthiraptera)

Supergroup F Wolbachia bacteria parasitise lice (Insecta: Phthiraptera)

The bacterial catalase from filarial DNA preparations derives from common pseudomonad contaminants and not from Wolbachia endosymbionts

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The bacterial catalase from filarial DNA preparations derives from common pseudomonad contaminants and not from Wolbachia endosymbionts