NDLI: Immunoprotection of chickens against Eimeria acervulina by recombinant α-tubulin protein

Content Provider	Springer Nature Link
Author	Ding, Jun Bao, Weichao Liu, Qun Yu, Qi Abdille, M. H. Wei, Zheng
Copyright Year	2008
Abstract	Tubulins are heterodimeric molecules responsible for the polymerization of microtubules in apicomplexan parasites. The α-tubulin, a subcellular structural protein of Eimeria acervulina, was cloned and expressed in Escherichia coli as an α-tubulin-GST fusion protein. Immunogenicity of the recombinant protein was studied in chickens by subcutaneous injection of 50, 100, or 150 μg of the protein with or without Freund’s adjuvant. Immunization with 150 μg α-tubulin-GST protein in combination with Freund’s adjuvant conferred partial protection against E. acervulina oocyst challenge, as shown by a 36% reduction in oocyst shedding, a marked decrease in intestinal lesion score and a significant increase in body weight gain in comparison with the nonimmunized controls. The results suggest that α-tubulin protein may be used as an effective vaccine antigen for the control of Eimeria infection.
Starting Page	1133
Ending Page	1140
Page Count	8
File Format	PDF
ISSN	09320113
Journal	Parasitology Research
Volume Number	103
Issue Number	5
e-ISSN	14321955
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-07-17
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Immunology Microbiology Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary Parasitology Insect Science

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Immunoprotection of chickens against Eimeria acervulina by recombinant α-tubulin protein