NDLI: Kinetic analysis of antibody responses to Blastocystis hominis in sera and intestinal secretions of orally infected mice

Content Provider	Springer Nature Link
Author	Santos, Herbert J. Rivera, Windell L.
Copyright Year	2009
Abstract	The kinetics of antibody production against Blastocystis hominis, an emerging infectious protozoan parasite which causes intestinal disorder in humans and animals, was studied. Sera and intestinal secretions were collected from B. hominis-immunized Balb/C mice for 8 weeks. Flow cytometry was used to monitor the levels of immunoglobulins A (IgA), G (IgG), and M (IgM) from both types of biological samples. The kinetic profile derived from flow cytometry analysis revealed that IgM led the early immune action against B. hominis infection in immune sera while IgA was the predominant antibody isotype in intestinal secretions. Western blotting revealed an array of antigens recognized by both serum and intestinal secretion antibodies. Immunoreactive B. hominis soluble proteins with molecular weights ranging from 28.2 to 77.6 kDa were detected by serum antibodies and 15.1 to 117.5 kDa by secretory antibodies. These antigens may be cytoplasmic or membrane-bound as determined through indirect fluorescent antibody test. Moreover, two immunogens (39.8 and 77.6 kDa) were commonly recognized by serum antibodies, one (70.8 kDa) by secretory antibodies and two (55.0 and 56.2 kDa) by both serum and secretory antibodies, suggesting a possible target in the further understanding of B. hominis pathogenicity, discovery of virulence factors, and development of immunology-based diagnostic protocols and alternative modes of treatment.
Starting Page	1303
Ending Page	1310
Page Count	8
File Format	PDF
ISSN	09320113
Journal	Parasitology Research
Volume Number	105
Issue Number	5
e-ISSN	14321955
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-07-14
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Immunology Microbiology Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary Parasitology Insect Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Pathogenic potential of Blastocystis hominis in laboratory mice

Antibody responses to Toxoplasma gondii in sera, intestinal secretions, and milk from orally infected mice and characterization of target antigens.

Encystation—survival of Blastocystis hominis in immunocompetent mice abdomen cavity

Urinary hyaluronidase activity in rats infected with Blastocystis hominis—evidence for invasion?

In vivo encystation of Blastocystis hominis

Evidence of plasmotomy in Blastocystis hominis

Association of Blastocystis hominis genetic subtypes with urticaria

Detection of Blastocystis hominis: a controversial human pathogen

Pathophysiological variability of different genotypes of human Blastocystis hominis Egyptian isolates in experimentally infected rats

Kinetic analysis of antibody responses to Blastocystis hominis in sera and intestinal secretions of orally infected mice

Similar Documents

Pathogenic potential of Blastocystis hominis in laboratory mice

Antibody responses to Toxoplasma gondii in sera, intestinal secretions, and milk from orally infected mice and characterization of target antigens.

Encystation—survival of Blastocystis hominis in immunocompetent mice abdomen cavity

Urinary hyaluronidase activity in rats infected with Blastocystis hominis—evidence for invasion?

In vivo encystation of Blastocystis hominis

Evidence of plasmotomy in Blastocystis hominis

Association of Blastocystis hominis genetic subtypes with urticaria

Detection of Blastocystis hominis: a controversial human pathogen

Pathophysiological variability of different genotypes of human Blastocystis hominis Egyptian isolates in experimentally infected rats

Kinetic analysis of antibody responses to Blastocystis hominis in sera and intestinal secretions of orally infected mice